Kim Y S, Bahn K N, Hah C K, Gang H I, Ha Y L
Division of Applied Life Sciences, Graduated School, and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 660-701, Korea.
J Food Sci. 2008 Jan;73(1):T16-20. doi: 10.1111/j.1750-3841.2007.00585.x.
Anticarcinogenic activity of medicinal herbs (Artemisia capillaris, Taxus cuspidata, Anthriscus sylveatris, and Curcuma longa) was examined for 7,12-dimethylbenz[a]anthracene (DMBA)-induced mouse skin carcinogenesis. Four types of solvent fractions (hexane, chloroform, ethyl acetate, and butanol) were prepared from the methanolic extract of medicinal herbs. The cytotoxicity and anticarcinogenic activities of solvent fractions were examined for mouse leukemia L1210 cancer cells and for female ICR mouse epidermal carcinogenesis induced by DMBA, respectively. The chloroform fraction of Artemisia capillaris, Taxus cuspidata, and Anthriscus sylveatris was more toxic to L1210 cells than other solvent fractions. The chloroform fraction of Artemisia capillaris markedly reduced the number of tumors/mouse and tumor incidence relative to that of other medicinal herbs tested. Major active chemical constituents in the chloroform fraction of Artemisia capillaries were found to be camphor, 1-borneol, coumarin, and achillin when analyzed by TLC and GC-MS. These results suggest that Artemisia capillaris was the most effective anticarcinogenic medicinal herb for DMBA-induced mouse epidermal carcinogenesis among 4 medicinal herbs tested, and the effect might be attributed to chemical compounds of camphor, 1-borneol, coumarin, and achillin.
研究了药用植物（茵陈蒿、东北红豆杉、峨参和姜黄）对7,12-二甲基苯并[a]蒽（DMBA）诱导的小鼠皮肤癌发生的抗癌活性。从药用植物的甲醇提取物中制备了四种溶剂提取物（己烷、氯仿、乙酸乙酯和丁醇）。分别检测了溶剂提取物对小鼠白血病L1210癌细胞和DMBA诱导的雌性ICR小鼠表皮癌发生的细胞毒性和抗癌活性。茵陈蒿、东北红豆杉和峨参的氯仿提取物对L1210细胞的毒性比其他溶剂提取物更大。相对于其他受试药用植物，茵陈蒿的氯仿提取物显著减少了每只小鼠的肿瘤数量和肿瘤发生率。通过薄层色谱法（TLC）和气相色谱-质谱联用（GC-MS）分析发现，茵陈蒿氯仿提取物中的主要活性化学成分是樟脑、1-冰片、香豆素和紫花前胡内酯。这些结果表明，在受试的4种药用植物中，茵陈蒿是对DMBA诱导的小鼠表皮癌发生最有效的抗癌药用植物，其效果可能归因于樟脑、1-冰片、香豆素和紫花前胡内酯等化合物。
