The Regional Center for Mycology and Biotechnology, Al-Azhar University, Nasr City, Cairo 11787, Egypt.
Faculty of Science (Girls Branch), Al-Azhar University, Nasr City, Cairo, Egypt.
ScientificWorldJournal. 2020 Jul 1;2020:3591276. doi: 10.1155/2020/3591276. eCollection 2020.
Gastric diseases are increasing with the infection of Late stages of infection lead to peptic ulcer and gastric carcinoma. infects people within different stages of their life, especially childhood, causing severe diarrhea; it infects around two-thirds of the world population. Due to bacterial resistance against standard antibiotic, a new strategy is needed to impede infections. Plants provide highly varied structures with antimicrobial use which are unlikely to be synthesized in laboratories. A special feature of higher plants is their ability to produce a great number of organic chemicals of high structural diversity, the so-called secondary metabolites. Twenty plants were screened to detect their antibacterial activities. Screening results showed that was the most efficient against . Fractionation pattern was obtained by column chromatography, while the purity test was done by thin-layer chromatography (TLC). The chemical composition of bioactive compound was characterized using GC-MS, nuclear magnetic resonance, and infrared analysis. Minimal inhibitory concentration (MIC) of the purified compound was 31.25 g/ml. Cytotoxicity assay on Vero cells was evaluated to be 497 g/ml. Furthermore, the purified bioactive compound activated human lymphocytes . The data presented here show that could potentially be used in modern applications aimed at the treatment or prevention of foodborne diseases.
胃部疾病随着感染的发生而增加。感染的后期阶段会导致消化性溃疡和胃癌。这种感染发生在人们生命的不同阶段，特别是在儿童时期，会导致严重的腹泻；它感染了世界上约三分之二的人口。由于细菌对标准抗生素的耐药性，需要一种新的策略来阻止感染。植物提供了高度多样化的结构，具有抗菌作用，这些结构不太可能在实验室中合成。高等植物的一个特殊特征是它们能够产生大量结构高度多样化的有机化学品，即所谓的次生代谢物。筛选了 20 种植物以检测其抗菌活性。筛选结果表明，对 的抑制效果最为显著。通过柱层析获得了分级模式，而通过薄层层析（TLC）进行了纯度测试。使用气相色谱-质谱联用仪、核磁共振和红外分析对生物活性化合物的化学组成进行了表征。纯化化合物的最小抑菌浓度（MIC）为 31.25 g/ml。对 Vero 细胞的细胞毒性测定为 497 g/ml。此外，纯化的生物活性化合物还激活了人淋巴细胞。这里呈现的数据表明，它可能有潜力用于现代应用，旨在治疗或预防食源性疾病。
