Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, New Delhi-110017, India.
Food Chem Toxicol. 2012 Sep;50(9):3126-32. doi: 10.1016/j.fct.2012.05.057. Epub 2012 Jun 9.
We investigated the potential of Tinospora cordifolia (TC) in treatment of diabetic retinopathy in STZ-induced rats due to its antihyperglycemic, angiogenic, antiinflammatory and antioxidant effects. The diabetic rats, treated for 24 weeks with TC extract (250 mg/kg), were evaluated for lenticular and fundus changes. Biochemical parameters were estimated and histopathological studies performed. TC significantly reduced blood glucose and glycated hemoglobin in treated rats. It prevented cataract development in treated group. Angiogenic markers VEGF and PKC increased in diabetic retina, which reduced significantly with TC. Anti-inflammatory parameters TNF-α and IL-1β elevated in diabetic group unlike that in treated group. TC also provided defense against depletion of antioxidant enzymes- glutathione and catalase. Histopathological studies revealed thickening of basement membrane of the retinal and glomerular vasculature of diabetic rat, but no basement membrane widening was seen in treated animals. Destruction of pancreatic islet structure was observed in diabetic group, but not in treated. Thus, TC reduces blood glucose and inhibits overexpression of angiogenic and inflammatory mediators, which are distinct markers of diabetic retinopathy. It also prevents retinal oxidative stress and restores antioxidant enzyme levels. These data provide evidence for the safety and potential effect of TC in the management of experimental diabetic retinopathy.
我们研究了三叶鬼针草（TC）在治疗链脲佐菌素（STZ）诱导的大鼠糖尿病性视网膜病变中的潜力，因为它具有降血糖、血管生成、抗炎和抗氧化作用。用 TC 提取物（250mg/kg）治疗 24 周的糖尿病大鼠，评估晶状体和眼底变化。评估生化参数并进行组织病理学研究。TC 可显著降低治疗大鼠的血糖和糖化血红蛋白。它可预防治疗组白内障的发生。血管生成标志物 VEGF 和 PKC 在糖尿病视网膜中增加，而 TC 可显著降低其水平。与治疗组不同，抗炎参数 TNF-α 和 IL-1β 在糖尿病组中升高。TC 还可以防止抗氧化酶-谷胱甘肽和过氧化氢酶的耗竭。组织病理学研究显示糖尿病大鼠视网膜和肾小球血管的基底膜增厚，但在治疗动物中未见基底膜增宽。糖尿病组观察到胰岛结构破坏，但在治疗组未观察到。因此，TC 可降低血糖并抑制血管生成和炎症介质的过度表达，这是糖尿病性视网膜病变的明显标志物。它还可以防止视网膜氧化应激并恢复抗氧化酶水平。这些数据为 TC 在治疗实验性糖尿病性视网膜病变中的安全性和潜在效果提供了证据。
