Ocular Pharmacology Laboratory, Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, University of Delhi, New Delhi, India.
J Ocul Pharmacol Ther. 2013 May;29(4):419-26. doi: 10.1089/jop.2012.0089. Epub 2012 Dec 10.
The present study was aimed to evaluate the retinoprotective effects of Moringa oleifera (MO) in Streptozotocin-induced diabetic rats.
The study was continued for 24 weeks and evaluated for inflammatory (tumor necrosis factor [TNF]-α and interleukin [IL]-1β, angiogenic (vascular endothelial growth factor [VEGF] and protein kinase C [PKC]-β) and antioxidant (Glutathione, Superoxide dismutase, and Catalase) parameters. Retinal leakage was checked by Fluorescein angiography (FA) and fundus photographs were evaluated for retinal vessel caliber (arteriolar and venular). Transmission electron microscopy was done to determine basement membrane (BM) thickness.
The results of the present study showed potential hypoglycemic and retinal antioxidant effects of MO. In the present study, a significant rise in the expression of retinal inflammatory (TNF-α and IL-1β) and angiogenic (VEGF and PKC-β) parameters was observed in diabetic retinae as compared to normal retinae. However, MO-treated retinae showed marked inhibition in the expression of inflammatory and angiogenic parameters. Further, in the present study, diabetic retinae showed dilated retinal vessels as compared to normal. However, MO-treated retinae showed marked prevention in the dilatation of retinal vessels. Fluorescein angiograms obtained from diabetic retinae showed leaky and diffused retinal vasculature. On the other hand, MO-treated retinae showed intact retinal vasculature. Further, results of the transmission electron microscopy study showed thickened capillary BM in the diabetic retina as compared to normal retinae. However, treatment with MO prevented thickening of capillary BM.
Our result suggests that MO may be useful in preventing diabetes induced retinal dysfunction.
本研究旨在评估辣木（Moringa oleifera，MO）对链脲佐菌素诱导的糖尿病大鼠的视网膜保护作用。
本研究持续进行了 24 周，评估了炎症（肿瘤坏死因子 [TNF]-α 和白细胞介素 [IL]-1β）、血管生成（血管内皮生长因子 [VEGF] 和蛋白激酶 C [PKC]-β）和抗氧化（谷胱甘肽、超氧化物歧化酶和过氧化氢酶）参数。通过荧光血管造影（FA）检查视网膜渗漏，并通过眼底照相评估视网膜血管口径（小动脉和小静脉）。进行透射电子显微镜检查以确定基底膜（BM）厚度。
本研究结果表明 MO 具有潜在的降血糖和视网膜抗氧化作用。在本研究中，与正常视网膜相比，糖尿病视网膜中观察到视网膜炎症（TNF-α 和 IL-1β）和血管生成（VEGF 和 PKC-β）参数的表达显著升高。然而，MO 处理的视网膜表现出炎症和血管生成参数表达的明显抑制。此外，在本研究中，与正常视网膜相比，糖尿病视网膜显示出扩张的视网膜血管。然而，MO 处理的视网膜显示出对视网膜血管扩张的明显预防作用。从糖尿病视网膜获得的荧光血管造影显示渗漏和弥漫性视网膜血管。另一方面，MO 处理的视网膜显示出完整的视网膜血管。此外，透射电子显微镜研究结果表明，糖尿病视网膜中的毛细血管 BM 比正常视网膜厚。然而，MO 治疗可防止毛细血管 BM 增厚。
我们的结果表明，MO 可能有助于预防糖尿病引起的视网膜功能障碍。
