Sanmenxia Central Hospital - Department of Ophthalmology - Sanmenxia - China.
King Saud University - College of Science - Department of Zoology - Riyadh - Saudi Arabia.
Acta Cir Bras. 2024 Feb 5;39:e390124. doi: 10.1590/acb390124. eCollection 2024.
To determine the effect of gallic acid or its combination with glibenclamide on some biochemical markers and histology of the cornea of streptozotocin (STZ) induced diabetic rats.
Following induction of diabetes, 24 male albino rats were divided into four groups of six rats each. Groups 1 and 2 (control and diabetic) received rat pellets and distilled water; group 3 (gallic acid) received rat pellets and gallic acid (10 mg/kg, orally) dissolved in the distilled water; and group 4 (gallic acid + glibenclamide) received rat pellets, gallic acid (10 mg/kg, orally), and glibenclamide (5 mg/kg, orally) dissolved in the distilled water. The treatments were administered for three months after which the rats were sacrificed after an overnight fast. Blood and sera were collected for the determination of biochemical parameters, while their eyes were excised for histology.
STZ administration to the rats induced insulin resistance, hyperglycemia, microprotenuria, loss of weight, oxidative stress, inflammation, and alteration of their cornea histology, which was abolished following supplementation with gallic acid or its combination with glibenclamide.
The study showed the potentials of gallic acid and glibenclamide in mitigating systemic complication and histological changes in the cornea of diabetic rats induced with STZ.
研究没食子酸或其与格列本脲联合应用对链脲佐菌素（STZ）诱导的糖尿病大鼠角膜某些生化标志物和组织学的影响。
诱导糖尿病后，将 24 只雄性白化大鼠分为四组，每组 6 只。第 1 组和第 2 组（对照组和糖尿病组）给予大鼠颗粒饲料和蒸馏水；第 3 组（没食子酸组）给予大鼠颗粒饲料和溶于蒸馏水的没食子酸（10mg/kg，口服）；第 4 组（没食子酸+格列本脲组）给予大鼠颗粒饲料、溶于蒸馏水的没食子酸（10mg/kg，口服）和格列本脲（5mg/kg，口服）。治疗持续 3 个月，然后大鼠禁食过夜后处死。采集血液和血清以测定生化参数，同时切除眼睛进行组织学检查。
STZ 给药导致大鼠胰岛素抵抗、高血糖、微量蛋白尿、体重减轻、氧化应激、炎症和角膜组织学改变，而给予没食子酸或其与格列本脲联合应用可消除这些改变。
该研究表明没食子酸和格列本脲具有减轻 STZ 诱导的糖尿病大鼠全身并发症和角膜组织学变化的潜力。
