Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tianthalay Road, Tha Kham, Bang Khuntian, Bangkok 10150, Thailand.
Biochem Biophys Res Commun. 2012 Jul 13;423(4):632-7. doi: 10.1016/j.bbrc.2012.05.151. Epub 2012 Jun 8.
Upon glucose depletion, a massive reprogramming of gene expression occurs in the yeast Saccharomyces cerevisiae for the use of alternate carbon sources such as the nonfermentable compounds ethanol and glycerol. This process is mediated by the master kinase Snf1 that controls the activity of various targets including the transcriptional regulators Cat8, Sip4 and Adr1. We have recently identified Rds2 as an additional player in this pathway. Here, we have performed genome-wide location analysis of Rds2 in cells grown in the presence of glycerol. We show that Rds2 binds to promoters of genes involved in gluconeogenesis, the glyoxylate shunt, and the TCA cycle as well as some genes encoding mitochondrial components or some involved in the stress response. Interestingly, we also detected Rds2 at the promoters of SIP4, ADR1 and HAP4 which encodes the limiting subunit of the Hap2/3/4/5 complex, a regulator of respiration. Strikingly, we observed an important overlap between the targets of Rds2 and Adr1. Finally, we provide a model to account for the complex interplay among these transcriptional regulators.
当葡萄糖耗尽时，酵母酿酒酵母会进行大规模的基因表达重编程，以利用替代碳源，如不可发酵的化合物乙醇和甘油。这个过程是由主激酶 Snf1 介导的，它控制着各种靶标的活性，包括转录调节因子 Cat8、Sip4 和 Adr1。我们最近发现 Rds2 是该途径中的另一个参与者。在这里，我们在细胞生长在甘油存在的情况下进行了 Rds2 的全基因组定位分析。我们表明 Rds2 结合到参与糖异生、乙醛酸支路和 TCA 循环的基因的启动子上，以及一些编码线粒体成分或一些参与应激反应的基因的启动子上。有趣的是，我们还在 SIP4、ADR1 和 HAP4 的启动子上检测到了 Rds2，HAP4 编码 Hap2/3/4/5 复合物的限制亚基，是呼吸调节因子。引人注目的是，我们观察到 Rds2 和 Adr1 的靶标之间存在重要的重叠。最后，我们提供了一个模型来解释这些转录调节因子之间的复杂相互作用。
