Department of Biological Sciences, University of New Orleans, New Orleans, LA 70148, USA.
Kudo Biotechnology, 117 Kendrick Street, Needham, MA 02494, USA.
Genes (Basel). 2024 Aug 27;15(9):1128. doi: 10.3390/genes15091128.
Multiple transcription factors in the budding yeast are required for the switch from fermentative growth to respiratory growth. The Hap2/3/4/5 complex is a transcriptional activator that binds to CCAAT sequence elements in the promoters of many genes involved in the tricarboxylic acid cycle and oxidative phosphorylation and activates gene expression. Adr1 and Cat8 are required to activate the expression of genes involved in the glyoxylate cycle, gluconeogenesis, and utilization of nonfermentable carbon sources. Here, we characterize the regulation and function of the zinc cluster transcription factor Gsm1 using Western blotting and reporter-gene analysis. is subject to glucose repression, and it requires a CCAAT sequence element for Hap2/3/4/5-dependent expression under glucose-derepression conditions. Genome-wide CHIP analyses revealed many potential targets. We analyzed 29 of them and found that , , , , and require both Gsm1 and Hap4 for optimal expression. , , , and play important roles in gluconeogenesis and utilization of two-carbon compounds, and they are known to be regulated by Cat8. overexpression in Δ mutant cells increases the expression of these target genes and suppresses growth defects in Δ mutants on lactate medium. We propose that Gsm1 and Cat8 have shared functions in gluconeogenesis and utilization of nonfermentable carbon sources and that Cat8 is the primary regulator.
在出芽酵母中，多个转录因子对于从发酵生长到呼吸生长的转变是必需的。Hap2/3/4/5 复合物是一种转录激活因子，它与许多参与三羧酸循环和氧化磷酸化的基因的启动子中的 CCAAT 序列元件结合，并激活基因表达。Adr1 和 Cat8 被要求激活参与乙醛酸循环、糖异生和非发酵碳源利用的基因的表达。在这里，我们使用 Western blot 和报告基因分析来描述锌簇转录因子 Gsm1 的调控和功能。在葡萄糖抑制条件下，是受葡萄糖抑制的，并且需要 CCAAT 序列元件才能在葡萄糖去抑制条件下依赖于 Hap2/3/4/5 的表达。全基因组 CHIP 分析揭示了许多潜在的靶标。我们分析了其中的 29 个，发现 、 、 、 和 都需要 Gsm1 和 Hap4 才能达到最佳表达。 、 、 和 在糖异生和利用二碳化合物中发挥重要作用，并且已知受 Cat8 调控。在 Δ 突变体细胞中过表达 会增加这些靶基因的表达，并抑制 Δ 突变体在乳酸培养基中的生长缺陷。我们提出 Gsm1 和 Cat8 在糖异生和利用非发酵碳源方面具有共同的功能，并且 Cat8 是主要的调节因子。
