Yuan Wenji, Zong Chen, Huang Yingzhi, Gao Ying, Shi Dongyan, Chen Changsong, Liu Liyue, Wang Jinfu
Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Stem Cell Res. 2012 Sep;9(2):110-23. doi: 10.1016/j.scr.2012.05.003. Epub 2012 May 18.
It has been verified that placenta contains multi-lineage mesenchymal stem cells (MSCs). We have used a time-gradient attachment method to isolate placenta-derived MSCs (PMSCs). The morphology, differentiation potential, immunogenicity and xenogenic reconstruction potential of these PMSCs were examined. The results showed that PMSCs isolated using the time-gradient attachment method showed higher potential of in vitro proliferation and multi-lineage differentiation. PMSCs isolated using the time-gradient attachment method showed a low immunogenicity. HLA-A gene fragment and no HLA-DR gene fragment were detected in PMSCs isolated using the time-gradient attachment method, and the mixed lymphocyte reaction (MLR) assay identified that these cells inhibited the proliferation of the allogeneic T-lymphocytes induced by PHA. The transplantation in calvaria of rats showed that PMSCs had the higher xenogenic reconstruction potential. Finally, the significance of PMSCs isolated using the time-gradient attachment method in experimental and clinical applications is discussed.
现已证实胎盘含有多谱系间充质干细胞（MSCs）。我们采用时间梯度贴壁法分离胎盘来源的间充质干细胞（PMSCs）。检测了这些PMSCs的形态、分化潜能、免疫原性和异种重建潜能。结果显示，采用时间梯度贴壁法分离的PMSCs具有更高的体外增殖和多谱系分化潜能。采用时间梯度贴壁法分离的PMSCs免疫原性较低。在采用时间梯度贴壁法分离的PMSCs中未检测到HLA - A基因片段且检测到无HLA - DR基因片段，混合淋巴细胞反应（MLR）试验表明这些细胞抑制了PHA诱导的同种异体T淋巴细胞的增殖。大鼠颅骨移植显示PMSCs具有更高的异种重建潜能。最后，讨论了采用时间梯度贴壁法分离的PMSCs在实验和临床应用中的意义。
