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使用近红外荧光成像对3D生物打印构建物中移植干细胞进行非侵入性体内监测。

Non-invasive in vivo monitoring of transplanted stem cells in 3D-bioprinted constructs using near-infrared fluorescent imaging.

作者信息

Kim Soon Hee, Kwon Jin Seon, Cho Jae Gu, Park Kate G, Lim Tae Hyeon, Kim Moon Suk, Choi Hak Soo, Park Chan Hum, Lee Sang Jin

机构信息

Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard Winston-Salem North Carolina USA.

Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University Chuncheon Republic of Korea.

出版信息

Bioeng Transl Med. 2021 Mar 26;6(2):e10216. doi: 10.1002/btm2.10216. eCollection 2021 May.

DOI:10.1002/btm2.10216
PMID:34027098
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8126817/
Abstract

Cell-based tissue engineering strategies have been widely established. However, the contributions of the transplanted cells within the tissue-engineered scaffolds to the process of tissue regeneration remain poorly understood. Near-infrared (NIR) fluorescence imaging systems have great potential to non-invasively monitor the transplanted cell-based tissue constructs. In this study, labeling mesenchymal stem cells (MSCs) using a lipophilic pentamethine indocyanine (CTNF127, emission at 700 nm) as a NIR fluorophore was optimized, and the CTNF127-labeled MSCs (NIR-MSCs) were printed embedding in gelatin methacryloyl bioink. The NIR-MSCs-loaded bioink showed excellent printability. In addition, NIR-MSCs in the 3D constructs showed high cell viability and signal stability for an extended period in vitro. Finally, we were able to non-invasively monitor the NIR-MSCs in constructs after implantation in a rat calvarial bone defect model, and the transplanted cells contributed to tissue formation without specific staining. This NIR-based imaging system for non-invasive cell monitoring in vivo could play an active role in validating the cell fate in cell-based tissue engineering applications.

摘要

基于细胞的组织工程策略已被广泛确立。然而，组织工程支架内移植细胞对组织再生过程的贡献仍知之甚少。近红外（NIR）荧光成像系统在无创监测基于移植细胞的组织构建体方面具有巨大潜力。在本研究中，优化了使用亲脂性五甲川吲哚菁（CTNF127，发射波长为700nm）作为近红外荧光团标记间充质干细胞（MSCs）的方法，并将CTNF127标记的MSCs（NIR-MSCs）打印嵌入甲基丙烯酰化明胶生物墨水中。负载NIR-MSCs的生物墨水表现出优异的可打印性。此外，三维构建体中的NIR-MSCs在体外长时间显示出高细胞活力和信号稳定性。最后，我们能够在大鼠颅骨缺损模型植入后无创监测构建体中的NIR-MSCs，并且移植细胞在无需特定染色的情况下促进了组织形成。这种基于近红外的体内无创细胞监测成像系统在验证基于细胞的组织工程应用中的细胞命运方面可以发挥积极作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/6d00acd819bb/BTM2-6-e10216-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/e840f9dfb257/BTM2-6-e10216-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/c61aab8d5bc6/BTM2-6-e10216-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/a55eda6c424e/BTM2-6-e10216-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/53bde81727c2/BTM2-6-e10216-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/c1cb1a71a344/BTM2-6-e10216-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/6d00acd819bb/BTM2-6-e10216-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/e840f9dfb257/BTM2-6-e10216-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/c61aab8d5bc6/BTM2-6-e10216-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/a55eda6c424e/BTM2-6-e10216-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/53bde81727c2/BTM2-6-e10216-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/c1cb1a71a344/BTM2-6-e10216-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fe/8126817/6d00acd819bb/BTM2-6-e10216-g005.jpg

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