Klochko Vitalii, Zelena Liubov, IVoychuk Sergei, Ostapchuk Andriy
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotnogo Str., Kyiv 03680, Ukraine.
J Gen Appl Microbiol. 2012;58(2):129-35. doi: 10.2323/jgam.58.129.
The phenotypic, chemotaxonomic and genetic peculiarities of 5 deep strains of Alteromonas macleodii (isolated from Adriatic and Ionian Sea water from a depth of 1,000-3,500 m) and 5 strains of the same species isolated from the surface layer of Aegean, Andaman, Black Sea and Atlantic Ocean water near the British shore have been studied. Electron microscopy has shown that the deep strains' cells were, on average, two times longer (2.1±0.2×0.7±0.1 µm) than the surface strains' (1.1±0.1×0.6±0.1 µm). Using fatty acid analysis (particularly the mono-unsaturated C16:1 and C18:1 fatty acids contents) the deep and surface isolates were clearly separated into two clusters. Distinctions between them were also found in different lectin binding capacity, which is probably determined by the structure of their extracellular polysaccharide matrix. Analysis of the results of PCR with primers to repeated nucleotide sequences revealed a higher level of genetic polymorphism in surface strains in comparison with the deep isolates. This division was confirmed by the cluster analysis method though it was not as clear as in the fatty acids analysis. The described peculiarities are probably reflective of specific conditions in which A. macleodii strains live on the surface or in the depth of the world's oceans.
对5株深海麦克劳德氏交替单胞菌菌株（从亚得里亚海和爱奥尼亚海1000 - 3500米深处的海水中分离得到）以及5株从爱琴海、安达曼海、黑海表层海水和英国海岸附近大西洋表层海水中分离得到的同物种菌株的表型、化学分类学和遗传学特性进行了研究。电子显微镜观察显示，深海菌株的细胞平均长度（2.1±0.2×0.7±0.1微米）是表层菌株（1.1±0.1×0.6±0.1微米）的两倍左右。通过脂肪酸分析（特别是单不饱和C16:1和C18:1脂肪酸含量），深海菌株和表层菌株被清晰地分为两个簇。它们在不同的凝集素结合能力方面也存在差异，这可能由其细胞外多糖基质的结构决定。用针对重复核苷酸序列的引物进行PCR分析结果显示，与深海菌株相比，表层菌株的遗传多态性水平更高。尽管聚类分析方法不如脂肪酸分析那样清晰，但这种划分也得到了该方法的证实。所描述的这些特性可能反映了麦克劳德氏交替单胞菌菌株在世界海洋表层或深处生存的特定条件。
