Ramachandran Rajesh, Reifler Aaron, Wan Jin, Goldman Daniel
Department of Biological Chemistry, Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, MI, USA.
Methods Mol Biol. 2012;884:129-40. doi: 10.1007/978-1-61779-848-1_8.
The Cre-loxP recombination system is widely used as a genetic tool to achieve conditional gene expression and for lineage tracing. Though extensively used in mice, this technology has only recently been applied to zebrafish. Here we describe Cre-loxP methodology for conditional expression of transgenes in zebrafish and their use in lineage tracing Müller glia as they undergo cellular reprogramming and proliferation to repair damaged retinal circuitry following mechanical injury. This methodology can be used for conditional gene expression and lineage tracing at any stage of development and in any cell type.
Cre-loxP重组系统作为一种遗传工具被广泛用于实现条件性基因表达和谱系追踪。尽管该技术在小鼠中已被广泛应用，但直到最近才应用于斑马鱼。在此，我们描述了用于在斑马鱼中条件性表达转基因的Cre-loxP方法，以及在机械损伤后缪勒胶质细胞进行细胞重编程和增殖以修复受损视网膜回路时用于谱系追踪的方法。该方法可用于发育的任何阶段和任何细胞类型的条件性基因表达和谱系追踪。
