Department of Pharmaceutical Sciences, Faculty of Pharmacy, Nutrition and Health Sciences, University of Calabria, Italy.
Biol Res. 2011;44(3):213-8. Epub 2011 Nov 7.
The present study shows for the first time the phenolic composition and the in vitro properties (antioxidant and inhibition of nitric oxide production) of Hypericum calabricum Sprengel collected in Italy. The content of hypericins (hypericin and pseudohypericin), hyperforin, flavonoids (rutin, hyperoside, isoquercetrin, quercitrin, quercetin and biapigenin) and chlorogenic acid of H. calabricum, have been determined. The ethyl acetate fraction from the aerial parts of H. calabricum exhibited activity against the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) with IC50 value of 1.6 jig/ml. The test for inhibition of nitric oxide (NO) production was performed using the murine monocytic macrophage cell line RAW 264.7. The ethyl acetate fraction had significant activity with an IC50 value of 102 jig/ml and this might indicate that it would have an anti-inflammatory effect in vivo.
本研究首次展示了在意大利采集的贯叶金丝桃（Hypericum calabricum Sprengel）的酚类成分和体外特性（抗氧化和抑制一氧化氮产生）。测定了贯叶金丝桃中的金丝桃素（金丝桃素和伪金丝桃素）、贯叶连翘素、类黄酮（芦丁、金丝桃苷、异槲皮苷、槲皮苷、槲皮素和双白杨素）和绿原酸的含量。贯叶金丝桃地上部分的乙酸乙酯部分对自由基 1,1-二苯基-2-苦基肼（DPPH）具有活性，IC50 值为 1.6μg/ml。使用鼠单核巨噬细胞 RAW 264.7 细胞系进行抑制一氧化氮（NO）产生的测试。乙酸乙酯部分具有显著的活性，IC50 值为 102μg/ml，这表明它在体内可能具有抗炎作用。
