Department of Plastic and Reconstructive Surgery, Perugia University, Perugia, Italy.
Cell Prolif. 2013 Apr;46(2):193-202. doi: 10.1111/cpr.12020.
Our interest continues in discovering phytocomplexes from medicinal plants with phototoxic activity against human melanoma cells; thus the aim of the present study was to assess antioxidant, anti-inflammatory and phototoxic activity of Hypericum perforatum L. subsp. perforatum, and relate these properties to the plant's chemical composition.
Components of H. perforatum subsp. perforatum were extracted by hydroalcoholic solution and chemical profiles of preparations (HyTE-3) performed by HPTLC. Linoleic acid peroxidation and DPPH tests were used to assess antioxidant activity, while MTT assay allowed evaluation of anti-proliferative activity with respect to A375 human melanoma cells after irradiation with UVA dose, 1.8 J/cm(2) . Inhibition of nitric oxide production of macrophages was also investigated.
HyTE-3 indicated better antioxidant activity with β-carotene bleaching test in comparison to DPPH assay (IC50 = 0.89 μg/ml); significant phototoxicity in A375 cells at 78 μg/ml concentration resulted in cell destruction of 50%. HyTE-3 caused significant dose-related inhibition of nitric oxide production in murine monocytic macrophage cell line RAW 264.7 with IC50 value of 342 μg/ml.
The H. perforatum subsp. perforatum-derived product was able to suppress proliferation of human malignant melanoma A375 cells; extract together with UVA irradiation enhanced phototoxicity. This biological activity of antioxidant effects was combined with inhibition of nitric oxide production.
我们一直致力于从具有光毒性的药用植物中发现植物复合物，以对抗人类黑色素瘤细胞；因此，本研究的目的是评估贯叶连翘亚种贯叶连翘的抗氧化、抗炎和光毒性活性，并将这些特性与植物的化学成分相关联。
用醇水溶液提取贯叶连翘亚种贯叶连翘的成分，并通过 HPTLC 进行制剂（HyTE-3）的化学分析。用亚油酸过氧化和 DPPH 试验评估抗氧化活性，而 MTT 试验允许在 1.8 J/cm²UVA 剂量照射后评估对 A375 人黑色素瘤细胞的抗增殖活性。还研究了巨噬细胞一氧化氮产生的抑制作用。
与 DPPH 试验相比，HyTE-3 在β-胡萝卜素漂白试验中显示出更好的抗氧化活性（IC50 = 0.89 μg/ml）；在 78 μg/ml 浓度下，HyTE-3 对 A375 细胞表现出显著的光毒性，导致 50%的细胞破坏。HyTE-3 对鼠单核巨噬细胞系 RAW 264.7 中一氧化氮的产生有显著的剂量依赖性抑制作用，IC50 值为 342 μg/ml。
贯叶连翘亚种贯叶连翘的衍生产品能够抑制人恶性黑色素瘤 A375 细胞的增殖；提取物与 UVA 照射一起增强了光毒性。这种抗氧化作用的生物活性与抑制一氧化氮的产生相结合。
