Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, Beutenberg Campus, 07745 Jena, Germany.
Phytochem Anal. 2013 Jan-Feb;24(1):41-6. doi: 10.1002/pca.2377. Epub 2012 Jun 12.
In the plant kingdom, flaxseed (Linum usitatissimum L.) is the richest source of secoisolariciresinol diglucoside (SDG), which is of great interest because of its potential health benefits for human beings. The information about the kinetics of SDG formation during flaxseed development is rare and incomplete.
In this study, a reversed-phase high-performance liquid chromatography-diode array detection (HPLC-DAD) method was developed to quantify SDG and coniferin, a key biosynthetic precursor of SDG in flaxseed.
Seeds from different developmental stages, which were scaled by days after flowering (DAF), were harvested. After alkaline hydrolysis, the validated HPLC method was applied to determine SDG and coniferin concentrations of flaxseed from different developing stages.
Coniferin was found in the entire capsule as soon as flowering started and became undetectable 20 DAF. SDG was detected 6 DAF, and the concentration increased until maturity. On the other hand, the SDG amount in a single flaxseed approached the maximum around 25 DAF, before desiccation started. Concentration increase between 25 DAF and 35 DAF can be attributed to corresponding seed weight decrease.
The biosynthesis of coniferin is not synchronous with that of SDG. Hence, the concentrations of SDG and coniferin change during flaxseed development.
在植物王国中，亚麻籽（Linum usitatissimum L.）是芝麻木脂素二葡萄糖苷（SDG）含量最丰富的来源，由于其对人类健康的潜在益处，因此备受关注。关于亚麻籽发育过程中 SDG 形成动力学的信息很少且不完整。
本研究开发了一种反相高效液相色谱-二极管阵列检测（HPLC-DAD）方法，用于定量测定 SDG 和 SDG 的关键生物合成前体松柏苷。
收获不同发育阶段（以开花后天数（DAF）为单位）的种子。经过碱性水解后，应用经验证的 HPLC 方法测定不同发育阶段亚麻籽中的 SDG 和松柏苷浓度。
开花后一出现，整个蒴果中就发现了松柏苷，20 DAF 时检测不到。6 DAF 时检测到 SDG，其浓度一直增加到成熟。另一方面，在开始干燥之前，单个亚麻籽中的 SDG 含量在 25 DAF 左右达到最大值。25 DAF 和 35 DAF 之间的浓度增加可归因于相应的种子重量减少。
松柏苷的生物合成与 SDG 不同步。因此，亚麻籽发育过程中 SDG 和松柏苷的浓度会发生变化。
