Johnsson P, Kamal-Eldin A, Lundgren L N, Aman P
Department of Food Science and Department of Chemistry, Swedish University of Agricultural Sciences (SLU), Box 7051, 750 07 Uppsala, Sweden.
J Agric Food Chem. 2000 Nov;48(11):5216-9. doi: 10.1021/jf0005871.
A method was developed for the analysis of secoisolariciresinol diglucoside (SDG) in flaxseeds. The analytical method involves extraction of defatted flaxseed flour with dioxane/ethanol, aqueous base-hydrolysis, solid-phase purification of an SDG-containing fraction, and quantitative analysis by high-performance liquid chromatography (HPLC). Pure SDG was isolated from flaxseed using different column chromatographic methods and used, as external standard, for the calibration of the method and for quantification. The method was then applied to study the variation in SDG content in flaxseeds from 14 cultivars grown in Sweden and 15 cultivars grown in Denmark. The SDG content varied between 11.7 and 24.1 mg/g in defatted flaxseed flour and between 6.1 and 13.3 mg/g in whole flaxseeds.
开发了一种分析亚麻籽中开环异落叶松脂素二葡萄糖苷（SDG）的方法。该分析方法包括用二氧六环/乙醇提取脱脂亚麻籽粉、碱水水解、含SDG馏分的固相纯化以及通过高效液相色谱（HPLC）进行定量分析。使用不同的柱色谱方法从亚麻籽中分离出纯SDG，并用作外标，用于方法校准和定量。然后将该方法应用于研究瑞典种植的14个品种和丹麦种植的15个品种亚麻籽中SDG含量的变化。脱脂亚麻籽粉中SDG含量在11.7至24.1毫克/克之间，全亚麻籽中SDG含量在6.1至13.3毫克/克之间。
