塞尔维亚商业葡萄酒中反式和顺式白藜芦醇的测定。

Determination of trans- and cis-resveratrol in Serbian commercial wines.

作者信息

Cvejic Jelena M, Djekic Sanja V, Petrovic Aleksandar V, Atanackovic Milica T, Jovic Slobodan M, Brceski Ilija D, Gojkovic-Bukarica Ljiljana C

机构信息

Laboratory for Pharmaceutical and Natural Products Analysis, Department of Pharmacy, Faculty of Medicine, Hajduk Veljkova 3, University of Novi Sad, 21000 Novi Sad, Serbia.

出版信息

J Chromatogr Sci. 2010 Mar;48(3):229-34. doi: 10.1093/chromsci/48.3.229.

DOI:10.1093/chromsci/48.3.229

PMID:20223091

Abstract

Resveratrol (trans-3,5,4'-trihydroxystilbene) is a phytoalexin produced by grapevines in response to fungal infection, particularly to Botrytis cinerea. It has been shown that it possess various biological effects such as prevention of cardiovascular diseases and anti-inflammatory and anticancerogenic properties. Red wines are a primary source of resveratrol. Although a number of investigations have focused on the determination of resveratrol in wines of different countries, there is no similar study about the wines produced in Serbia. As authors are aware, the only study concerning resveratrol content in wine in the Balkan region was conducted in Greece. In this study, the trans- and cis-resveratrol content in samples obtained from 18 commercial Serbian wines (10 red, 7 white, and 1 rose) were analyzed. Analyses were performed after solid-phase extraction by high-performance liquid chromatography with a diode array detection system using an RP-C(18) column with gradient elution [solvent A: acetonitrile-acetic acid-water (20:2:78 v/v), solvent B: acetonitrile-acetic acid-water (90:2:8 v/v)]. Detection of trans- and cis-resveratrol was performed on 306 and 286 nm, respectively. It was clearly established that there was a presence of trans-resveratrol isomers in all analyzed wines (0.11-1.69 mg/L) except in one white wine. Cis-resveratrol was present in 12 from 18 samples in different amounts (0.12-1.49 mg/L).

摘要

白藜芦醇（反式-3,5,4'-三羟基茋）是葡萄藤在受到真菌感染，特别是灰葡萄孢菌感染时产生的一种植保素。研究表明，它具有多种生物学效应，如预防心血管疾病以及抗炎和抗癌特性。红酒是白藜芦醇的主要来源。尽管许多研究都集中在测定不同国家葡萄酒中的白藜芦醇含量，但尚未有针对塞尔维亚产葡萄酒的类似研究。据作者所知，巴尔干地区唯一一项关于葡萄酒中白藜芦醇含量的研究是在希腊进行的。在本研究中，对从18种塞尔维亚商业葡萄酒（10种红葡萄酒、7种白葡萄酒和1种玫瑰葡萄酒）中获取的样品中的反式和顺式白藜芦醇含量进行了分析。分析是在固相萃取后，使用配备二极管阵列检测系统的高效液相色谱仪，采用RP-C(18)柱进行梯度洗脱[溶剂A：乙腈-乙酸-水（20:2:78 v/v），溶剂B：乙腈-乙酸-水（90:2:8 v/v）]来进行的。反式和顺式白藜芦醇的检测分别在306 nm和286 nm波长下进行。结果明确表明，除了一种白葡萄酒外，所有分析的葡萄酒中均存在反式白藜芦醇异构体（0.11 - 1.69 mg/L）。18个样品中有12个含有不同含量的顺式白藜芦醇（0.12 - 1.49 mg/L）。