Hossain Md Sohrab, Balakrishnan Venugopal, Rahman Nik Norulaini Nik Ab, Sarker Md Zaidul Islam, Kadir Mohd Omar Ab
Department of Environmental Technology, School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia.
Int J Environ Res Public Health. 2012 Mar;9(3):855-67. doi: 10.3390/ijerph9030855. Epub 2012 Mar 9.
A steam autoclave was used to sterilize bacteria in clinical solid waste in order to determine an alternative to incineration technology in clinical solid waste management. The influence of contact time (0, 5, 15, 30 and 60 min) and temperature (111 °C, 121 °C and 131 °C) at automated saturated steam pressure was investigated. Results showed that with increasing contact time and temperature, the number of surviving bacteria decreased. The optimum experimental conditions as measured by degree of inactivation of bacteria were 121 °C for 15 minutes (min) for Gram negative bacteria, 121 °C and 131 °C for 60 and 30 min for Gram positive bacteria, respectively. The re-growth of bacteria in sterilized waste was also evaluated in the present study. It was found that bacterial re-growth started two days after the inactivation. The present study recommends that the steam autoclave cannot be considered as an alternative technology to incineration in clinical solid waste management.
为了确定临床固体废物管理中焚烧技术的替代方法,使用蒸汽高压灭菌器对临床固体废物中的细菌进行灭菌。研究了在自动饱和蒸汽压力下接触时间(0、5、15、30和60分钟)和温度(111°C、121°C和131°C)的影响。结果表明,随着接触时间和温度的增加,存活细菌数量减少。以细菌灭活程度衡量的最佳实验条件分别为:革兰氏阴性菌在121°C下处理15分钟,革兰氏阳性菌在121°C下处理60分钟、在131°C下处理30分钟。本研究还评估了灭菌后废物中细菌的再生长情况。发现细菌在灭活两天后开始重新生长。本研究建议,在临床固体废物管理中,蒸汽高压灭菌器不能被视为焚烧的替代技术。