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[CSF RT-PCR standardization in pediatric patients with enteroviral central nervous system infection].

作者信息

García-Elorriaga Guadalupe, Esparza-García Amalia, Méndez-Rojas Claudia, del Rey-Pineda Guillermo, González-Bonilla César

机构信息

Unidad de Investigación en Inmunología e Infectología, Hospital de Infectología Dr. Federico Méndez, Centro Médico Nacional La Raza, IMSS, México, DF.

出版信息

Rev Invest Clin. 2012 Jan-Feb;64(1):59-66.

PMID:22690530
Abstract

OBJECTIVE

We standardized the RT-PCR panviral CSF and determined its applicability in detecting acute enterovirus infection in the central nervous system in children under 15 years.

MATERIAL AND METHODS

RT-PCR was performed directly in CSF samples of 10 pediatric patients with suspected CNS infection and 9, with different conditions of the central nervous system.

RESULTS

80% (8/10) of RT-PCR samples were positive for enterovirus in patients with suspected CNS infection and no sample was positive in patients with different ailments.

CONCLUSIONS

Since enteroviruses are among the main etiologies of pediatric encephalitis, RT-PCR could be particularly useful for rapid detection in CSF.

摘要

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