Department of Operative Dentistry and Periodontology, Martin-Luther University Halle-Wittenberg, Halle, Germany.
J Clin Periodontol. 2012 Aug;39(8):699-706. doi: 10.1111/j.1600-051X.2012.01909.x. Epub 2012 Jun 12.
The aim of this analysis was to evaluate the importance of genetic variants of TNFα for the severity of periodontal disease and periodontal risk factors with respect to periodontal risk factors in a cohort of coronary patients.
A total of 942 consecutive patients with angiographic proven coronary heart disease were prospectively included in the study entitled "Periodontitis and Its Microbiological Agents as Prognostic Factors in Patients With Coronary Heart Disease" (ClinicalTrials.gov identifier:NCT01045070).
After including of patients, an extensive periodontal examination also involving PCR-sampling for 11 periodontal bacteria was performed. In this subanalysis, single nucleotide polymorphisms (SNPs) c.-308G>A, c.-238G>A and haplotypes for TNFα were analysed by CTS-PCR-SSP Tray kit (Heidelberg, Germany).
The AG+AA genotype of SNP c.-238G>A of TNFα gene was associated with the amount of clinical attachment loss in patients with coronary heart disease in multivariate regression analysis. Moreover, Prevotella intermedia occurred more frequently in carriers who were positive for the AG+AA genotype and A-allele of SNP c.-308G>A in bivariate and multivariate analyses. Furthermore, only in bivariate analyses significant associations of genetic variants of TNFα with intensified bleeding on probing and with higher plasma level of interleukin 6 could be shown.
Genetic variants of TNFα gene, namely c.-308G>A and c.-238G>A, are associated with periodontal conditions in patients with coronary heart disease.
本分析旨在评估 TNFα 基因的遗传变异对冠心病患者牙周病严重程度和牙周危险因素的重要性。
共纳入 942 例经血管造影证实的冠心病连续患者，前瞻性纳入题为“牙周炎及其微生物作为冠心病患者预后因素”的研究（ClinicalTrials.gov 标识符：NCT01045070）。
在纳入患者后，对患者进行了广泛的牙周检查，还涉及 11 种牙周细菌的 PCR 采样。在这项亚分析中，通过 CTS-PCR-SSP Tray 试剂盒（德国海德堡）分析 TNFα 基因的单核苷酸多态性（SNP）c.-308G>A、c.-238G>A 和 TNFα 单倍型。
TNFα 基因 SNP c.-238G>A 的 AG+AA 基因型与冠心病患者的临床附着丧失量在多变量回归分析中相关。此外，在双变量和多变量分析中，双歧杆菌属中间型在 SNP c.-308G>A 的 AG+AA 基因型和 A-等位基因的携带者中更为常见。此外，只有在双变量分析中，TNFα 基因遗传变异与探查时出血增加和白细胞介素 6 血浆水平升高之间存在显著关联。
TNFα 基因的遗传变异，即 c.-308G>A 和 c.-238G>A，与冠心病患者的牙周状况有关。
