Armingohar Zahra, Jørgensen Jørgen J, Kristoffersen Anne K, Schenck Karl, Dembic Zlatko
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway;
Department of Vascular Surgery, Oslo University Hospital, Aker, Norway.
J Oral Microbiol. 2015 Feb 19;7:26051. doi: 10.3402/jom.v7.26051. eCollection 2015.
Chronic periodontitis (CP), atherosclerotic and aortic aneurysmal vascular diseases (VD) are chronic inflammatory conditions with multifactorial etiologies, including involvement of predisposing genetic factors. In a previous study, polymorphisms in the gene for the anti-inflammatory interleukin-1 receptor antagonist were associated with CP in patients with VD.
This study investigates whether polymorphisms in the gene for the anti-inflammatory interleukin-10 (IL10) could be related to CP in the same manner.
Seventy-two patients with VD of whom 35 had CP were genotyped for single nucleotide polymorphisms (SNPs) in the IL10 -592 (rs1800872), -819 (rs1800871), and -1,082 (rs1800896) gene by Taqman rtPCR method and by DNA sequencing.
The C alleles and C/C genotypes of IL10 -592 and IL10 -819 frequencies were significantly higher, while the frequencies of the IL10 -592 (C/A) and IL10 -819 (C/T) heterozygote genotypes were significantly lower in the VD group with CP compared to those without CP. The IL10 haplotype ATA frequency (-1,082, -819, -592) showed a trend to a significant difference between the two groups indicating protection against CP.
Taken together, our findings suggest an independent association of genetic polymorphisms in the IL-10 gene locus with CP in patients with VD. Development of CP and the implications on vascular disease emphasize the importance of early detection and adequate treatment of periodontitis among these patients.
慢性牙周炎（CP）、动脉粥样硬化和主动脉瘤性血管疾病（VD）是具有多因素病因的慢性炎症性疾病，包括易感遗传因素的参与。在先前的一项研究中，抗炎性白细胞介素-1受体拮抗剂基因的多态性与VD患者的CP相关。
本研究调查抗炎性白细胞介素-10（IL10）基因的多态性是否可能以相同方式与CP相关。
对72例VD患者（其中35例患有CP）采用Taqman rtPCR方法和DNA测序对IL10基因-592（rs1800872）、-819（rs1800871）和-1082（rs1800896）位点的单核苷酸多态性（SNP）进行基因分型。
与无CP的VD组相比，患有CP的VD组中IL10 -592和IL10 -819的C等位基因及C/C基因型频率显著更高，而IL10 -592（C/A）和IL10 -819（C/T）杂合子基因型频率显著更低。IL10单倍型ATA频率（-1082，-819，-592）在两组之间显示出显著差异的趋势，表明对CP具有保护作用。
综上所述，我们的研究结果表明IL-10基因座中的遗传多态性与VD患者的CP存在独立关联。CP的发生及其对血管疾病的影响强调了在这些患者中早期检测和充分治疗牙周炎的重要性。
