Yim H S, Chye F Y, Tan C T, Ng Y C, Ho C W
Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University 56000 Kuala Lumpur, Malaysia.
Malays J Nutr. 2010 Aug;16(2):281-91. Epub 2010 Aug 15.
Pleurotus ostreatus better known as oyster mushroom is widely cultivated and consumed as food in Malaysia. The present study aims to assess the antioxidative potential and total phenolic content of P. ostreatus aqueous extract. The antioxidant activities were evaluated against DPPH and ABTS radical-scavenging activity, ferric-reducing antioxidant power (FRAP) and β-carotene-linoleate bleaching assay, and the Folin-Ciocalteu method for total phenolic content (TPC). The DPPH and ABTS radical-scavenging activity was found to be 63.20% and 87.29% respectively; antioxidant activity using FRAP at 1.45 mM FE/100g and β-carotenelinoleate bleaching assay was 83.51%, while the TPC was found to be 798.55 mg GAE/100g. These antioxidant activities were compared to synthetic antioxidant, BHA and ascorbic acid. Ascorbic acid showed highest scavenging effects on DPPH and ABTS radical, followed by P. ostreatus and BHA (at maximum safety limit). The ferric reducing power of P. ostreatus was significantly higher than BHA and ascorbic acid. The antioxidant activity as assessed in β-carotene-linoleate bleaching assay was found to be higher in BHA compared to P. ostreatus. The aqueous extract of P. ostreatus was found to respond differently in antioxidant assays. The antioxidative activity of the aqueous extract of P. ostreatus correlated with its total phenolic content. Generally, the antioxidant activities of P. ostreatus' aqueous extract are comparable to that of BHA and ascorbic acid to a certain extent.
平菇（又称蚝菇）在马来西亚被广泛种植并作为食物食用。本研究旨在评估平菇水提取物的抗氧化潜力和总酚含量。通过DPPH和ABTS自由基清除活性、铁还原抗氧化能力（FRAP）和β-胡萝卜素-亚油酸酯漂白试验评估抗氧化活性，并采用福林-酚法测定总酚含量（TPC）。发现DPPH和ABTS自由基清除活性分别为63.20%和87.29%；使用FRAP测得的抗氧化活性为1.45 mM FE/100g，β-胡萝卜素-亚油酸酯漂白试验的抗氧化活性为83.51%，而TPC为798.55 mg GAE/100g。将这些抗氧化活性与合成抗氧化剂丁基羟基茴香醚（BHA）和抗坏血酸进行比较。抗坏血酸对DPPH和ABTS自由基的清除效果最高，其次是平菇和BHA（在最大安全限量下）。平菇的铁还原能力显著高于BHA和抗坏血酸。在β-胡萝卜素-亚油酸酯漂白试验中评估的抗氧化活性，BHA高于平菇。发现平菇水提取物在抗氧化试验中的反应不同。平菇水提取物的抗氧化活性与其总酚含量相关。一般来说，平菇水提取物的抗氧化活性在一定程度上与BHA和抗坏血酸相当。
