van Neste D
Unit of Occupational Dermatology, Catholic University of Louvain, Brussels, Belgium.
Dermatologica. 1990;181(3):197-201.
In a series of previous experiments, we showed that the inflammatory response associated with skin barrier function damage induced by sodium laurylsulfate (SLS) was correlated with the concentration of SLS and with the duration of application under occlusion of the surfactant. In this experiment, we measured accumulation of unbound water that occurred when occlusive contact was maintained during a short period of time between the skin and the probe head of an electrometric device. Accumulation of unbound water was expressed as area under the curve (sum of increments of skin capacitance during a 45-second period of time; AUCC). For comparative purposes, we also measured transepidermal water loss (TEWL). Both skin function parameters were established for each individual skin site before and 24 h after removal of SLS patches (10, 5, 1 and 0% control solution under occlusion for 48 h). After SLS insult, AUCC and TEWL were increased, and changes were related to SLS concentration (p less than 0.0002). There was a linear correlation between AUCC and log TEWL (p less than 0.0002). On normal skin before application of SLS both parameters were also correlated (p less than 0.04). The regression line between AUCC and log TEWL of normal skin differed however from that observed on SLS-pretreated sites. This indicates that electrometric data are able to simultaneously characterize changes in skin hydration, a rather static parameter, and changes in the amount of unbound water accumulation (AUCC), a dynamic parameter which proved to reflect TEWL.
在一系列先前的实验中，我们表明，与月桂醇硫酸酯钠（SLS）诱导的皮肤屏障功能损伤相关的炎症反应与SLS的浓度以及表面活性剂在封闭条件下的应用持续时间相关。在本实验中，我们测量了在皮肤与静电测量装置的探头之间短时间保持封闭接触时发生的未结合水的积累。未结合水的积累用曲线下面积表示（45秒时间段内皮肤电容增量的总和；AUCC）。为了进行比较，我们还测量了经表皮水分流失（TEWL）。在去除SLS贴片（10%、5%、1%和0%对照溶液在封闭条件下处理48小时）之前和之后24小时，为每个个体皮肤部位建立了这两个皮肤功能参数。SLS损伤后，AUCC和TEWL增加，且变化与SLS浓度相关（p<0.0002）。AUCC与log TEWL之间存在线性相关性（p<0.0002）。在应用SLS之前的正常皮肤上，这两个参数也相关（p<0.04）。然而，正常皮肤的AUCC与log TEWL之间的回归线与在SLS预处理部位观察到的不同。这表明静电测量数据能够同时表征皮肤水合作用（一个相当静态的参数）的变化以及未结合水积累量（AUCC，一个动态参数，已证明可反映TEWL）的变化。
