Uoshima K, Handelman B, Cooper L F
Clinical Investigations Patient Care Branch, National Institutes of Dental Research, Bethesda, MD 20892.
Biochem Biophys Res Commun. 1993 Dec 30;197(3):1388-95. doi: 10.1006/bbrc.1993.2631.
A gene encoding the rat heat shock protein 27 protein was isolated and characterized. The gene is composed of three exons and two introns. The proximal 5' untranslated region of this gene contains consensus heat shock elements, multiple SP1 sites, and basal regulatory elements including a CCAAT box and a TATA box. Comparison of this heat shock gene with the murine heat shock protein 25 and human heat shock protein 27 genes revealed the conserved presence of a putative heat shock element in the first intron of these mammalian small M(r) heat shock protein genes. While preliminary expression studies indicate the promoter directs heat shock induced expression of heat shock protein 27, the functional significance of the intronic heat shock element to the expression of this gene remains to be determined.
分离并鉴定了一个编码大鼠热休克蛋白27的基因。该基因由三个外显子和两个内含子组成。该基因近端的5'非翻译区包含共有热休克元件、多个SP1位点以及包括CCAAT框和TATA框在内的基础调控元件。将该热休克基因与小鼠热休克蛋白25基因和人类热休克蛋白27基因进行比较，发现在这些哺乳动物小分子量热休克蛋白基因的第一个内含子中保守存在一个推定的热休克元件。虽然初步表达研究表明该启动子指导热休克诱导的热休克蛋白27的表达，但内含子热休克元件对该基因表达的功能意义仍有待确定。
