Konícek J, Daraselia G Y, Konícková-Radochová M, Lasík J, Wurst M, Koza T
Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Folia Microbiol (Praha). 1990;35(5):394-401. doi: 10.1007/BF02821408.
Mutant strains of Mycobacterium sp. V-649 producing highly mucous colonies on a solid cultivation medium were prepared after treatment with N-methyl-N'-nitro-N-nitrosoguanidine and production of the exocellular polysaccharide was tested. The strains were cultivated in media with suitable sugar sources under submerged conditions. It was found that Mycobacterium sp. V-649/15 produces a maximum of 15-19% polymer after a 5-6-d cultivation. Gas chromatography indicated that the exocellular polysaccharide produced by this strain is of glucan type.
用N-甲基-N'-硝基-N-亚硝基胍处理后，制备了在固体培养基上产生高度粘液状菌落的分枝杆菌属V-649突变株，并测试了细胞外多糖的产生。这些菌株在淹没条件下于含有合适糖源的培养基中培养。发现分枝杆菌属V-649/15在5-6天培养后产生的聚合物最多可达15-19%。气相色谱表明该菌株产生的细胞外多糖为葡聚糖类型。
