肉桂链霉菌原生质体的制备及完整细胞的再生
Preparation of protoplasts and regeneration of intact cells of Streptomyces cinnamonensis.
作者信息
Jandová Z, Tichý P
机构信息
Department of General Microbiology, Czechoslovak Academy of Sciences, Prague.
出版信息
Folia Microbiol (Praha). 1990;35(5):456-9. doi: 10.1007/BF02821416.
PMID:2269446
Abstract
Protoplasts were prepared and intact cells were regenerated in Streptomyces cinnamonensis--a monensin producer--to make genetic manipulations with this strain possible. 70-80% of protoplasts were formed and up to 90% of them could regenerate into intact cells.
摘要
制备了肉桂链霉菌（莫能菌素产生菌）的原生质体，并使其再生出完整细胞，从而使对该菌株进行基因操作成为可能。形成了70%-80%的原生质体，其中高达90%能够再生为完整细胞。
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