• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

肉桂链霉菌原生质体的制备及完整细胞的再生

Preparation of protoplasts and regeneration of intact cells of Streptomyces cinnamonensis.

作者信息

Jandová Z, Tichý P

机构信息

Department of General Microbiology, Czechoslovak Academy of Sciences, Prague.

出版信息

Folia Microbiol (Praha). 1990;35(5):456-9. doi: 10.1007/BF02821416.

DOI:10.1007/BF02821416
PMID:2269446
Abstract

Protoplasts were prepared and intact cells were regenerated in Streptomyces cinnamonensis--a monensin producer--to make genetic manipulations with this strain possible. 70-80% of protoplasts were formed and up to 90% of them could regenerate into intact cells.

摘要

制备了肉桂链霉菌(莫能菌素产生菌)的原生质体,并使其再生出完整细胞,从而使对该菌株进行基因操作成为可能。形成了70%-80%的原生质体,其中高达90%能够再生为完整细胞。

相似文献

1
Preparation of protoplasts and regeneration of intact cells of Streptomyces cinnamonensis.肉桂链霉菌原生质体的制备及完整细胞的再生
Folia Microbiol (Praha). 1990;35(5):456-9. doi: 10.1007/BF02821416.
2
Mutant strains of Streptomyces cinnamonensis protoplasts. Cultural and physiological conditions.肉桂链霉菌原生质体的突变菌株。培养和生理条件。
Folia Microbiol (Praha). 1991;36(1):42-8. doi: 10.1007/BF02935821.
3
Protoplast isolation and regeneration in Streptomyces clavuligerus.棒状链霉菌原生质体的分离与再生
J Gen Microbiol. 1989 Aug;135(8):2289-97. doi: 10.1099/00221287-135-8-2289.
4
A comparative study of protoplast preparation and regeneration in Streptomyces rimosus and Streptomyces lividans.龟裂链霉菌和变铅青链霉菌原生质体制备与再生的比较研究
Microbiologica. 1986 Jul;9(3):387-92.
5
[Protoplasts of lincomycin producer Streptomyces roseolus in genetic manipulations].[林可霉素产生菌玫瑰链霉菌原生质体在基因操作中的应用]
Antibiot Khimioter. 1991 Oct;36(10):3-5.
6
[Preparation and regeneration of protoplasts of the strain Streptomyces hygroscopicus 155].[吸水链霉菌155菌株原生质体的制备与再生]
Antibiot Khimioter. 1992 Dec;37(12):24-6.
7
Protoplast formation and regeneration in Streptomyces flavopersicus.黄色嗜热放线菌原生质体的形成与再生
Lett Appl Microbiol. 1993 Apr;16(4):217-9. doi: 10.1111/j.1472-765x.1993.tb01401.x.
8
Formation and reversion of Streptomycete protoplasts: cultural condition and morphological study.链霉菌原生质体的形成与再生:培养条件及形态学研究
J Gen Microbiol. 1974 Feb;80(2):389-400. doi: 10.1099/00221287-80-2-389.
9
Formation, regeneration, and transformation of protoplasts of Streptomyces diastatochromogenes 1628.龟裂链霉菌1628原生质体的形成、再生及转化
Folia Microbiol (Praha). 2014 Mar;59(2):93-7. doi: 10.1007/s12223-013-0271-5. Epub 2013 Jul 31.
10
[Cytological study of early stages of regeneration of protoplasts of fungi and Streptomyces].[真菌和链霉菌原生质体再生早期阶段的细胞学研究]
Antibiot Khimioter. 1988 Mar;33(3):196-200.

引用本文的文献

1
Properties of Saccharopolyspora erythraea strains after protoplast regeneration.原生质体再生后糖多孢红霉菌株的特性
Folia Microbiol (Praha). 2005;50(1):13-8. doi: 10.1007/BF02931288.
2
Transformation of Streptomyces lincolnensis protoplasts with plasmid vectors.用质粒载体转化林肯链霉菌原生质体。
Folia Microbiol (Praha). 1992;37(3):181-7. doi: 10.1007/BF02933144.

本文引用的文献

1
Protoplast fusion in Streptomyces: conditions for efficient genetic recombination and cell regeneration.链霉菌中的原生质体融合:高效基因重组和细胞再生的条件
J Gen Microbiol. 1981 Nov;127(1):137-46. doi: 10.1099/00221287-127-1-137.
2
An improved method for the preparation of Streptomycetes and Micromonospora protoplasts.一种制备链霉菌和小单孢菌原生质体的改进方法。
J Antibiot (Tokyo). 1983 Feb;36(2):184-6. doi: 10.7164/antibiotics.36.184.
3
Formation and reversion of Streptomycete protoplasts: cultural condition and morphological study.
链霉菌原生质体的形成与再生:培养条件及形态学研究
J Gen Microbiol. 1974 Feb;80(2):389-400. doi: 10.1099/00221287-80-2-389.
4
New antibiotic-producing streptomycetes, selected by antibiotic resistance as a marker. I. New antibiotic production generated by protoplast fusion treatment between Streptomyces griseus and S. tenjimariensis.通过抗生素抗性筛选出的新型产抗生素链霉菌。一、灰色链霉菌和天城链霉菌原生质体融合处理产生的新抗生素生产。
J Antibiot (Tokyo). 1985 Jan;38(1):58-63. doi: 10.7164/antibiotics.38.58.
5
Transformation of Streptomyces erythraeus.红霉素链霉菌的转化
J Antibiot (Tokyo). 1986 Sep;39(9):1304-13. doi: 10.7164/antibiotics.39.1304.
6
A comparative study of protoplast preparation and regeneration in Streptomyces rimosus and Streptomyces lividans.龟裂链霉菌和变铅青链霉菌原生质体制备与再生的比较研究
Microbiologica. 1986 Jul;9(3):387-92.