Stewart W W, Kerr M A
Department of Pathology, University of Dundee, Ninewells Hospital and Medical School, U.K.
Immunology. 1990 Nov;71(3):328-34.
Heat or chemically aggregated IgA or IgG stimulated degranulation of neutrophils with comparable efficiency. The same aggregates induced a neutrophil respiratory burst which could be measured by lucigenin-enhanced chemiluminescence. Serum IgA1 or IgA2 coated onto microtitre plates were both capable of inducing a respiratory burst in neutrophils, as was secretory IgA1 or secretory IgA2. All bursts were of similar size for a given concentration of IgA and were greater than the burst elicited by an equivalent concentration of IgG. For each subclass of IgA the respiratory bursts were dependent on their density on the opsonized surface. Since monomeric and dimeric forms present in secretory IgA preparations both elicit a respiratory burst in neutrophils, secretory component and J chain cannot block the receptor binding site on the Fc region. The potential of secretory IgA to act as an opsonin might have important consequences on mucosal surfaces where the availability of complement components is limited.
热聚合或化学聚合的IgA或IgG以相当的效率刺激中性粒细胞脱颗粒。相同的聚集体诱导中性粒细胞呼吸爆发，这可以通过光泽精增强的化学发光来测量。包被在微量滴定板上的血清IgA1或IgA2都能够诱导中性粒细胞呼吸爆发，分泌型IgA1或分泌型IgA2也是如此。对于给定浓度的IgA，所有爆发的规模相似，且大于同等浓度IgG引发的爆发。对于IgA的每个亚类，呼吸爆发取决于它们在调理表面上的密度。由于分泌型IgA制剂中存在的单体和二聚体形式均能引发中性粒细胞呼吸爆发，因此分泌成分和J链不能阻断Fc区域上的受体结合位点。分泌型IgA作为调理素的潜力可能会对补体成分有限的粘膜表面产生重要影响。
