Tran H T, Metcalf D, Cheers C
Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
Immunology. 1990 Nov;71(3):377-82.
Two lines of transgenic mice carrying the gene for granulocyte-macrophage colony-stimulating factor (GM-CSF) produce vastly increased numbers of macrophages with abundant foamy cytoplasm resembling classical activated macrophages. Cells from both lines were negative for myeloperoxidase, a bactericidal enzyme found in monocytes as well as neutrophils, but not mature macrophages. Cells from the so called 'male line' produced greatly increased levels of oxygen degradation products in response to phagocytosis, compared with cells from the 'female line' or from normal littermates. The ability of the cells to phagocytose and lyse the intracellular bacterium Listeria monocytogenes was tested in vitro using radiolabelled organisms. Although the cells from transgenic mice were more highly phagocytic than cells from normal littermates, cells from either line were no more efficient than normal at lysing the bacteria they had phagocytosed. Nevertheless, because of the high phagocytic rate, more bacteria were exposed to lysis in the cells of transgenic mice, and the final outcome was a higher rate of bacteriolysis.
携带粒细胞-巨噬细胞集落刺激因子（GM-CSF）基因的两系转基因小鼠产生了数量大幅增加的巨噬细胞，这些巨噬细胞具有丰富的泡沫状细胞质，类似于经典活化巨噬细胞。两系细胞的髓过氧化物酶均呈阴性，髓过氧化物酶是一种存在于单核细胞和中性粒细胞而非成熟巨噬细胞中的杀菌酶。与“雌性系”或正常同窝小鼠的细胞相比，来自所谓“雄性系”的细胞在吞噬作用下产生的氧降解产物水平大幅增加。使用放射性标记的生物体在体外测试了细胞吞噬和裂解细胞内细菌单核细胞增生李斯特菌的能力。尽管转基因小鼠的细胞比正常同窝小鼠的细胞具有更高的吞噬活性，但两系细胞在裂解它们吞噬的细菌方面并不比正常细胞更有效。然而，由于吞噬率高，转基因小鼠细胞中有更多细菌受到裂解，最终结果是细菌裂解率更高。
