Centre for Research in Biosciences, Department of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK.
J Gen Virol. 2012 Sep;93(Pt 9):2046-2056. doi: 10.1099/vir.0.043331-0. Epub 2012 Jun 13.
The bacteriophage vB_SenS-Ent1 (Ent1) is a member of the family Siphoviridae of tailed bacteriophages and infects a broad range of serovars of the enteric pathogen Salmonella enterica. The virion particle is composed of an icosahedral head 64 nm in diameter and a flexible, non-contractile tail of 116 × 8.5 nm possessing terminal fibres. The adsorption rate constant at 37 °C is 6.73 × 10(-9) ml min(-1). Latent and eclipse periods are 25 and 20 min, respectively, and the burst size is 35 progeny particles per cell after 35 min at 37 °C. Sequencing revealed a circularly permuted, 42 391 bp dsDNA genome containing 58 ORFs organized into four major transcriptional units. Comparisons with the genome sequences of other bacteriophages revealed a high level of nucleotide sequence identity and shared orthologous proteins with the Salmonella phages SETP3, SE2 and KS7 (SS3e) and the Escherichia phages K1G, K1H, K1ind1 and K1ind3.
噬菌體 vB_SenS-Ent1 (Ent1) 是尾噬菌体科 Siphoviridae 的成員，感染腸道致病菌沙門氏菌屬的廣泛血清型。病毒粒子由直徑 64nm 的二十面體頭和 116×8.5nm 的柔韌、非收縮尾組成，具有末端纖維。在 37°C 下的吸附速率常數為 6.73×10(-9)ml min(-1)。潛伏期和隱蔽期分別為 25 和 20 分鐘，爆發量為 37°C 下 35 分鐘後每個細胞產生 35 個子代粒子。序列分析揭示了一條圓形排列的 42391bp 的 dsDNA 基因組，包含 58 個 ORF，分佈在四個主要的轉錄單元中。與其他噬菌體的基因組序列比較表明，核苷酸序列同一性水平很高，並與沙門氏菌噬菌體 SETP3、SE2 和 KS7 (SS3e) 以及大腸桿菌噬菌體 K1G、K1H、K1ind1 和 K1ind3 具有共享的同源蛋白。
