Tung Chuan-Wei, Alvarado-Martínez Zabdiel, Tabashsum Zajeba, Aditya Arpita, Biswas Debabrata
Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA.
Biological Sciences Program-Molecular and Cellular Biology, University of Maryland, College Park, MD 20742, USA.
Foods. 2023 Feb 13;12(4):797. doi: 10.3390/foods12040797.
() is the most common foodborne pathogen worldwide, leading to massive economic loss and a significant burden on the healthcare system. The primary source of remains contaminated or undercooked poultry products. Considering the number of foodborne illnesses with multiple antibiotic resistant , new controlling approaches are necessary. Bacteriophage (phage) therapies have emerged as a promising alternative to controlling bacterial pathogens. However, the limitation on the lysis ability of most phages is their species-specificity to the bacterium. has various serovars, and several major serovars are involved in gastrointestinal diseases in the USA. In this study, bacteriophage-1252 (phage-1252) was isolated and found to have the highest lytic activity against multiple serovars of , including Typhimurium, Enteritidis, Newport, Heidelberg, Kentucky, and Gallinarum. Whole-genome sequencing analysis revealed phage-1252 is a novel phage strain that belongs to the genus Duplodnaviria in the Myoviridae family, and consists of a 244,421 bp dsDNA, with a G + C content of 48.51%. Its plaque diameters are approximately 2.5 mm to 0.5 mm on the agar plate. It inhibited Enteritidis growth after 6 h. The growth curve showed that the latent and rise periods were approximately 40 min and 30 min, respectively. The burst size was estimated to be 56 PFU/cell. It can stabilize and maintain original activity between 4 °C and 55 °C for 1 h. These results indicate that phage-1252 is a promising candidate for controlling multiple serovars in food production.
（某菌）是全球最常见的食源性病原体，导致巨大的经济损失和医疗系统的重大负担。该菌的主要来源仍然是受污染或未煮熟的家禽产品。考虑到具有多重抗生素抗性的食源性疾病数量，新的控制方法是必要的。噬菌体疗法已成为控制细菌病原体的一种有前途的替代方法。然而，大多数噬菌体裂解能力的限制在于它们对细菌的种特异性。（某菌）有多种血清型，在美国，几种主要血清型与胃肠道疾病有关。在本研究中，分离出噬菌体-1252（phage-1252），发现它对该菌的多种血清型具有最高的裂解活性，包括鼠伤寒血清型、肠炎血清型、纽波特血清型、海德堡血清型、肯塔基血清型和鸡伤寒血清型。全基因组测序分析表明，phage-1252是一种新型噬菌体菌株，属于肌尾噬菌体科双链DNA病毒目双链DNA病毒纲的双病毒属，由一条244,421 bp的双链DNA组成，G + C含量为48.51%。其在琼脂平板上的噬菌斑直径约为2.5毫米至0.5毫米。6小时后它抑制了肠炎血清型（某菌）的生长。生长曲线表明，潜伏期和上升期分别约为40分钟和30分钟。裂解量估计为56个噬菌斑形成单位/细胞。它可以在4°C至55°C之间稳定并保持原始活性1小时。这些结果表明，phage-1252是食品生产中控制该菌多种血清型的有前途的候选者。
