Ebenezar J, Pu Yang, Wang W B, Liu C H, Alfano R R
PG & Research Department of Physics, Jamal Mohamed College, Tiruchirappalli, Tamilnadu 620020, India.
Appl Opt. 2012 Jun 1;51(16):3642-9. doi: 10.1364/AO.51.003642.
Stokes shift spectroscopy (S3) is an emerging approach toward cancer detection. The goal of this paper is to evaluate the diagnostic potential of the S3 technique for the detection and characterization of normal and cancerous prostate tissues. Pairs of cancerous and normal prostate tissue samples were taken from each of eight patients. Stokes shift spectra were measured by simultaneously scanning both the excitation and emission wavelengths while keeping a fixed wavelength interval Δλ=20 nm between them. The salient features of this technique are the highly resolved emission peaks and significant spectral differences between the normal and cancerous prostate tissues, as observed in the wavelength region of 250 to 600 nm. The Stokes shift spectra of cancerous and normal prostate tissues revealed distinct peaks around 300, 345, 440, and 510 nm, which are attributed to tryptophan, collagen, NADH, and flavin, respectively. To quantify the spectral differences between the normal and cancerous prostate tissues, two spectral ratios were computed. The findings revealed that both ratio parameters R1=I297/I345 and R2=I307/I345 were excellent diagnostic ratio parameters giving 100% specificity and 100% sensitivity for distinguishing cancerous tissue from the normal tissue. Our results demonstrate that S3 is a sensitive and specific technique for detecting cancerous prostate tissue.
斯托克斯位移光谱法（S3）是一种新兴的癌症检测方法。本文的目的是评估S3技术在检测和表征正常及癌性前列腺组织方面的诊断潜力。从八名患者身上分别采集了癌性和正常前列腺组织样本。通过同时扫描激发波长和发射波长来测量斯托克斯位移光谱，同时保持它们之间固定的波长间隔Δλ = 20 nm。在250至600 nm波长区域观察到，该技术的显著特征是发射峰高度分辨，以及正常和癌性前列腺组织之间存在明显的光谱差异。癌性和正常前列腺组织的斯托克斯位移光谱在300、345、440和510 nm左右显示出明显的峰，分别归因于色氨酸、胶原蛋白、NADH和黄素。为了量化正常和癌性前列腺组织之间的光谱差异，计算了两个光谱比率。研究结果表明，比率参数R1 = I297/I345和R2 = I307/I345都是出色的诊断比率参数，在区分癌性组织和正常组织方面具有100%的特异性和100%的敏感性。我们的结果表明，S3是一种检测癌性前列腺组织的灵敏且特异的技术。
