Department of Medical Physics, Anna University, Chennai, India.
Department of Oral Medicine and Radiology, Meenakshi Ammal Dental College and Hospital, Chennai, India.
J Fluoresc. 2019 Mar;29(2):361-367. doi: 10.1007/s10895-018-02343-3. Epub 2019 Jan 23.
High incidence of oral cancer is primarily due to ongoing tobacco epidemic. In this work, synchronous luminescence spectroscopy (SLS) has been used to characterize and discriminate oral cancer tissue. Spectral deconvolution method is employed to compute the fluorescence intensity, peak wavelength, and full width half maxima for different endogenous fluorophores. The fluorescence measurements were made on 21 normal and 88 oral squamous cell carcinoma biopsy tissues. Besides, variations in relative concentration of collagen, NADH, and FAD, peak shifts and broadening of peaks are observed for tryptophan, NADH, and FAD, in oral cancer tissues indicating both biochemical and micro environmental changes at cellular level. Linear discriminant analysis showed that oral cancer tissue is discriminated with a sensitivity and specificity of 100% and 95.2% respectively.
口腔癌的高发主要是由于烟草的持续流行。在这项工作中，同步发光光谱（SLS）已被用于对口腔癌组织进行特征描述和区分。采用光谱反卷积方法计算不同内源性荧光团的荧光强度、峰值波长和半峰全宽。对 21 例正常和 88 例口腔鳞状细胞癌活检组织进行了荧光测量。此外，在口腔癌组织中观察到色氨酸、NADH 和 FAD 的相对浓度变化、峰位移和峰展宽，表明细胞水平的生化和微环境变化。线性判别分析表明，口腔癌组织的灵敏度和特异性分别为 100%和 95.2%。
