Hunan Academy of Inspection & Quarantine, Hunan Key Lab. of Science & Technology for Food Safety, Technogy Center of Hunan Entry-Exit Inspection & Quarantine Bureau of P. R. China, Changsha, P. R. China.
J Sep Sci. 2010 Jul;33(14):2109-14. doi: 10.1002/jssc.200900841.
A simple and sensitive method was developed for determination of illegal adulterants (yohimbine, sildenafil, vardenafil and tadalafil) in dietary supplements by HPLC-MS/MS. The separation was achieved on a C(18) column with the mobile phase consisting of acetonitrile and 0.1% acetic acid aqueous solution with a gradient elution at a flow rate of 0.5 mL/min. The analytes were quantified and identified by two characteristic transitions using the multiple-reaction monitoring mode. The recoveries of the analytes ranged from 77.5 to 109.3% with the RSD less than 8.1% (n=6). The method has been successfully applied to screen illegal adulterations of natural dietary supplements.
建立了一种高效液相色谱-串联质谱法用于检测保健品中非法添加物（育亨宾、西地那非、伐地那非和他达那非）的简单灵敏的方法。采用 C18 柱，以乙腈和 0.1%乙酸水溶液为流动相，梯度洗脱，流速为 0.5ml/min。采用多反应监测模式，以两个特征离子转换定量和确证分析物。分析物的回收率在 77.5%至 109.3%之间，相对标准偏差小于 8.1%（n=6）。该方法已成功应用于天然保健品中非法添加物的筛查。
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