INRA, UR1268 Biopolymères Interactions Assemblages, F-44316 Nantes, France.
Int J Biol Macromol. 2012 Nov;51(4):462-70. doi: 10.1016/j.ijbiomac.2012.05.024. Epub 2012 Jun 12.
The impact of genetic and fruit ripening on hemicelluloses fine structure was studied in twelve near isogenic lines of tomato fruits harboring firmness QTL. The sugar composition and the MALDI-TOF MS oligosaccharides profile after glucanase hydrolysis of the cell walls were determined from all green and red fruits pericarp tissue. MS profiles showed two major series of oligomers attributed to xyloglucan (XG) and glucomannan (GM) with minor peaks for xylan and ions attributed to galacto-oligomers. The oligosaccharides MS intensity varied significantly with the fruit genetic and ripening status. Correlations between MS intensity indicated structural regulations of both XG and GM structures with genetics and ripening. These results point to a region on the tomato chromosome 9 controlling cell wall galactose metabolism.
研究了 12 个近等基因系番茄果实中基因和果实成熟对半纤维素精细结构的影响，这些果实都含有硬度 QTL。从所有绿色和红色果实的果皮组织中确定了细胞壁经葡聚糖酶水解后的糖组成和 MALDI-TOF MS 寡糖谱。MS 图谱显示了两个主要的低聚糖系列，归因于木葡聚糖 (XG) 和葡甘露聚糖 (GM)，并有少量的木聚糖和归因于半乳糖低聚糖的离子峰。寡糖 MS 强度随果实遗传和成熟状态显著变化。MS 强度之间的相关性表明 XG 和 GM 结构的结构调控与遗传和成熟有关。这些结果表明，番茄染色体 9 上的一个区域控制着细胞壁半乳糖代谢。
