Nkouawa Agathe, Sako Yasuhito, Li Tiaoying, Chen Xingwang, Nakao Minoru, Yanagida Tetsuya, Okamoto Munehiro, Giraudoux Patrick, Raoul Francis, Nakaya Kazuhiro, Xiao Ning, Qiu Jiamin, Qiu Dongchuan, Craig Philip S, Ito Akira
Department of Parasitology, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, 078-8510, Hokkaido, Japan.
Parasitol Int. 2012 Dec;61(4):723-5. doi: 10.1016/j.parint.2012.06.001. Epub 2012 Jun 11.
In this study, we applied a loop-mediated isothermal amplification method for identification of human Taenia tapeworms in Tibetan communities in Sichuan, China. Out of 51 proglottids recovered from 35 carriers, 9, 1, and 41 samples were identified as Taenia solium, Taenia asiatica and Taenia saginata, respectively. Same results were obtained afterwards in the laboratory, except one sample. These results demonstrated that the LAMP method enabled rapid identification of parasites in the field surveys, which suggested that this method would contribute to the control of Taenia infections in endemic areas.
在本研究中,我们应用环介导等温扩增法对中国四川藏族社区的人体带绦虫进行鉴定。从35名携带者身上采集的51节孕节中,分别有9份、1份和41份样本被鉴定为猪带绦虫、亚洲带绦虫和牛带绦虫。除一份样本外,随后在实验室获得了相同的结果。这些结果表明,环介导等温扩增法能够在现场调查中快速鉴定寄生虫,这表明该方法将有助于在流行地区控制带绦虫感染。