Department of Chemistry, Korea University, Seoul 136-701, Korea.
J Am Chem Soc. 2013 Jun 19;135(24):9181-5. doi: 10.1021/ja403851p. Epub 2013 Jun 7.
A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer and a mitochondria-targeting unit, was synthesized. The sensor showed a direct linear relationship between the fluorescence intensity ratio of BODIPY to coumarin or the fluorescence lifetime ratio and the media viscosity, which allowed us to determine the average mitochondrial viscosity in living HeLa cells as ca. 62 cP (cp). Upon treatment with an ionophore, monensin, or nystatin, the mitochondrial viscosity was observed to increase to ca. 110 cP.
一种用于细胞线粒体的自校准双分体粘度传感器 1，由香豆素和硼二吡咯甲烷（BODIPY）组成，具有刚性苯基间隔基和线粒体靶向单元。该传感器显示出 BODIPY 与香豆素的荧光强度比或荧光寿命比与介质粘度之间的直接线性关系，这使我们能够确定活 HeLa 细胞中的平均线粒体粘度约为 62 cP（厘泊）。用离子载体莫能菌素或制霉菌素处理后，观察到线粒体粘度增加到约 110 cP。
