Genomics Research Center, Academia Sinica, Nankang, Taipei, Taiwan.
Anal Chem. 2012 Jul 3;84(13):5765-9. doi: 10.1021/ac301022n. Epub 2012 Jun 14.
Presented herein are the development of macromolecular ion accelerator (MIA) and the results obtained by MIA. This new instrument utilizes a consecutive series of planar electrodes for the purpose of facilitating stepwise acceleration. Matrix-assisted laser desorption/ionization (MALDI) is employed to generate singly charged macromolecular ions. A regular Z-gap microchannel plate (MCP) detector is mounted at the end of the accelerator to record the ion signals. In this work, we demonstrated the detection of ions with the mass-to-charge (m/z) ratio reaching 30,000,000. Moreover, we showed that singly charged biomolecular ions can be accelerated with the voltage approaching 1 MV, offering the evidence that macromolecular ions can possess much higher kinetic energy than ever before.
本文介绍了大分子离子加速器（MIA）的发展及其研究成果。该新型仪器采用连续的平面电极来实现逐步加速。基质辅助激光解吸/电离（MALDI）用于产生单电荷的大分子离子。在加速器的末端安装了一个常规的 Z 间隙微通道板（MCP）检测器来记录离子信号。在这项工作中，我们展示了对质荷比（m/z）高达 30000000 的离子的检测。此外，我们还表明，单电荷的生物分子离子可以在接近 1MV 的电压下被加速，这证明了大分子离子可以拥有比以往更高的动能。
