Philandrianos C, Gonnelli D, Andrac-Meyer L, Bruno M, Magalon G, Mordon S
Service de chirurgie plastique et maxillofacial, hôpital Nord de Marseille, chemin des Bourrely, 13915 Marseille cedex, France; Inserm U 703, 152, rue du Dr.-Yersin, 59120 Loos, France.
Service de chirurgie plastique et maxillofacial, hôpital Nord de Marseille, chemin des Bourrely, 13915 Marseille cedex, France.
Ann Chir Plast Esthet. 2014 Aug;59(4):246-52. doi: 10.1016/j.anplas.2012.05.001. Epub 2012 Jun 12.
Keloid scar is a proliferative healing dysfunction formed by an excessive build-up of collagen fibers on the dermis. It is responsible of aesthetic and functional disabilities. There is no ideal treatment and recurrence occurs very often. Keloid scars occur only to human, that's why animal model needs to be made to study this pathology and new treatments. Few models have been described using human keloid scars implanted into subcutaneous tissue of nude mice or rat. To allow study of topical and laser treatment we have developed a new animal model using human keloid scar fragment with epidermal and dermal tissue implanted into back of nude mice like a full thickness skin graft. Keloid fragments from five donors have been grafted onto 40 nudes mice. Macroscopic and microscopic studies have been made at day 28, 56, 84 and 112. We observed integration of the fragments in all cases. Hyalinized collagen bundles were observed in all implant biopsies confirming the stability of the keloid architecture within 112 days. This model is easily reproducible and allows the study of topical treatment and laser due to the accessibility of the keloid.
瘢痕疙瘩是一种增殖性愈合功能障碍，由真皮层胶原纤维过度堆积形成。它会导致美观和功能障碍。目前尚无理想的治疗方法，且复发很常见。瘢痕疙瘩仅发生于人类，因此需要建立动物模型来研究这种病理情况和新的治疗方法。很少有模型描述将人类瘢痕疙瘩植入裸鼠或大鼠的皮下组织。为了能够研究局部治疗和激光治疗，我们开发了一种新的动物模型，将带有表皮和真皮组织的人类瘢痕疙瘩碎片像全层皮肤移植一样植入裸鼠背部。来自五名捐赠者的瘢痕疙瘩碎片已移植到40只裸鼠身上。在第28天、56天、84天和112天进行了宏观和微观研究。我们观察到在所有情况下碎片均已整合。在所有植入活检中均观察到透明化的胶原束，证实了瘢痕疙瘩结构在112天内的稳定性。该模型易于复制，并且由于瘢痕疙瘩易于触及，因此可以用于研究局部治疗和激光治疗。
