Philandrianos Cécile, Bertrand Baptiste, Andrac-Meyer Lucile, Magalon Guy, Casanova Dominique, Kerfant Nathalie, Mordon Serge
Department of Plastic Surgery, AP-HM, Marseille, 13915, France.
Aix-Marseille University, Marseille, France.
Lasers Surg Med. 2015 Dec;47(10):798-806. doi: 10.1002/lsm.22428. Epub 2015 Oct 6.
A temperature increase can improve wound healing by activation of heat shock protein 70 and stimulation of fibroblasts. Since keloids are a dysfunction of collagen fiber synthesis and organization, this study aimed to evaluate if a 1,210 nm diode laser could have effects in a new animal model of keloid scars.
STUDY DESIGN/MATERIALS AND METHODS: A total of 39 nude mice were used for this study. Phototypes IV and V human keloids were grafted into their backs and after 1 month of healing, the mice were divided into four groups: Control, Laser, Resection, Resection/Laser. In the Laser group, the keloids were treated with a 1,210-nm diode-laser with the following parameters: 4 W; 10 seconds; fluence: 51 J/cm(2) ; spot: 18.9 × 3.7 mm(2) . In the Resection group, surgical intra-lesional excision was performed. In the Resection/Laser group, keloids were treated with the 1,210-nm laser-diode after surgical intra-lesional excision. Temperature measurements were made during the laser treatment. Clinical examination and histological study were performed on the day of treatment and 1 month, 2 months, and 3 months later.
Mean temperature measurement was of 44.8°C (42-48°) in the Laser groups. No healing complications or keloid proliferation was observed in any group. Keloid histologic characters were confirmed in all grafts. No histologic particularity was observed in the laser groups in comparison with the Control and Resection groups.
First, this keloid animal model appears to be adapted for laser study. Secondly, the 1,210-nm diode laser does not induce keloid thermal damage in vivo. Further studies with different 1,210-nm laser diode parameters should be performed in order to observe significant effects on keloids.
温度升高可通过激活热休克蛋白70和刺激成纤维细胞来促进伤口愈合。由于瘢痕疙瘩是胶原纤维合成和排列的功能障碍，本研究旨在评估1210纳米二极管激光对瘢痕疙瘩新动物模型是否有作用。
研究设计/材料与方法：本研究共使用了39只裸鼠。将IV型和V型人类瘢痕疙瘩移植到它们的背部，愈合1个月后，将小鼠分为四组：对照组、激光组、切除组、切除/激光组。在激光组中，用1210纳米二极管激光治疗瘢痕疙瘩，参数如下：4瓦；10秒；能量密度：51焦/平方厘米；光斑：18.9×3.7平方毫米。在切除组中，进行手术病灶内切除。在切除/激光组中，手术病灶内切除后用1210纳米激光二极管治疗瘢痕疙瘩。激光治疗期间进行温度测量。在治疗当天以及1个月、2个月和3个月后进行临床检查和组织学研究。
激光组的平均温度测量值为44.8°C（42-48°C）。所有组均未观察到愈合并发症或瘢痕疙瘩增殖。所有移植组织均证实有瘢痕疙瘩组织学特征。与对照组和切除组相比，激光组未观察到组织学特殊性。
首先，这种瘢痕疙瘩动物模型似乎适用于激光研究。其次，1210纳米二极管激光在体内不会引起瘢痕疙瘩热损伤。应使用不同的1210纳米激光二极管参数进行进一步研究，以观察对瘢痕疙瘩的显著效果。
