Yang Yang, He Xiaofeng, Li Yanhao, Feng Jianyu, Pang Huajin, Wang Jiangyun, Liu Qing
Department of Interventional Radiology, Southern Medical University, Guangzhou, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Jun;32(6):897-900.
To explore the role of transforming growth factor-β1 (TGF-β1) in intervertebral disc degeneration and its association with the pathological grading of disc degeneration.
Normal and degenerative intervertebral disc tissues were collected were classified into 5 grades of increasing degenerative changes. HE staining, immunohistochemistry, TUNEL staining and RT-PCR were used to detect the expression of TGF-β1 in the disc tissues.
Immunohistochemistry and RT-PCR showed positive expressions of TGF-β1 and Bcl-2 in normal disc tissues, where Bax was expressed at have a trace level. In the degenerative disc tissues, TGF-β1 expression increased with the pathological grades; the expression levels of TGF-β1 showed significant differences between degenerative and normal tissues and between grade IV and grade I disc tissues (P<0.01).
TGF-β1 is an important factor participating in the disc degeneration and its expression level is closely related to the pathological grade of degenerative discs.
探讨转化生长因子-β1（TGF-β1）在椎间盘退变中的作用及其与椎间盘退变病理分级的关系。
收集正常和退变的椎间盘组织，将退变组织分为退变程度递增的5个等级。采用苏木精-伊红（HE）染色、免疫组织化学、末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）染色及逆转录-聚合酶链反应（RT-PCR）检测椎间盘组织中TGF-β1的表达。
免疫组织化学和RT-PCR显示，正常椎间盘组织中TGF-β1和Bcl-2呈阳性表达，Bax呈微量表达。在退变的椎间盘组织中，TGF-β1表达随病理分级增加而升高；退变组织与正常组织之间以及IV级与I级椎间盘组织之间TGF-β1表达水平差异有统计学意义（P<0.01）。
TGF-β1是参与椎间盘退变的重要因素，其表达水平与退变椎间盘的病理分级密切相关。
