Department of Biomedical Engineering, W.J. Kolff Institute, University Medical Center Groningen and the University of Groningen, Groningen, The Netherlands.
Biofouling. 2012;28(6):583-91. doi: 10.1080/08927014.2012.698614.
The composition of microbial biofilms on silicone rubber facial prostheses was investigated and compared with the microbial flora on healthy and prosthesis-covered skin. Scanning electron microscopy showed the presence of mixed bacterial and yeast biofilms on and deterioration of the surface of the prostheses. Microbial culturing confirmed the presence of yeasts and bacteria. Microbial colonization was significantly increased on prosthesis-covered skin compared to healthy skin. Candida spp. were exclusively isolated from prosthesis-covered skin and from prostheses. Biofilms from prostheses showed the least diverse band-profile in denaturing gradient gel electrophoresis (DGGE) whereas prosthesis-covered skin showed the most diverse band-profile. Bacterial diversity exceeded yeast diversity in all samples. It is concluded that occlusion of the skin by prostheses creates a favorable niche for opportunistic pathogens such as Candida spp. and Staphylococcus aureus. Biofilms on healthy skin, skin underneath the prosthesis and on the prosthesis had a comparable composition, but the numbers present differed according to the microorganism.
研究了硅橡胶面部假体上微生物生物膜的组成，并将其与健康皮肤和假体覆盖皮肤的微生物菌群进行了比较。扫描电子显微镜显示，假体表面存在混合细菌和酵母生物膜，并出现了恶化。微生物培养证实了酵母和细菌的存在。与健康皮肤相比，假体覆盖的皮肤微生物定植明显增加。假体外皮肤和假体上仅分离出假丝酵母菌。来自假体的生物膜在变性梯度凝胶电泳（DGGE）中显示出最少的多样化条带谱，而假体覆盖的皮肤显示出最多的多样化条带谱。所有样本中，细菌多样性均超过酵母多样性。结论是，假体对皮肤的闭塞为假丝酵母菌和金黄色葡萄球菌等机会性病原体创造了有利的小生境。健康皮肤、假体下方皮肤和假体上的生物膜具有相似的组成，但根据微生物的不同，存在的数量也不同。
