(64)Cu-ATSM 和 (18)FDG PET 摄取及 (64)Cu-ATSM 放射自显影在自发性犬肿瘤中的应用：与 pimonidazole 缺氧免疫组化的比较。
(64)Cu-ATSM and (18)FDG PET uptake and (64)Cu-ATSM autoradiography in spontaneous canine tumors: comparison with pimonidazole hypoxia immunohistochemistry.
机构信息
Department of Radiation Oncology, Copenhagen University Hospital, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.
出版信息
Radiat Oncol. 2012 Jun 15;7:89. doi: 10.1186/1748-717X-7-89.
BACKGROUND
The aim of this study was to compare (64)Cu-diacetyl-bis(N(4)-methylsemicarbazone) ((64)Cu-ATSM) and (18)FDG PET uptake characteristics and (64)Cu-ATSM autoradiography to pimonidazole immunohistochemistry in spontaneous canine sarcomas and carcinomas.
METHODS
Biopsies were collected from individual tumors between approximately 3 and 25 hours after the intravenous injection of (64)Cu-ATSM and pimonidazole. (64)Cu-ATSM autoradiography and pimonidazole immunostaining was performed on sectioned biopsies. Acquired (64)Cu-ATSM autoradiography and pimonidazole images were rescaled, aligned and their distribution patterns compared. (64)Cu-ATSM and (18)FDG PET/CT scans were performed in a concurrent study and uptake characteristics were obtained for tumors where available.
RESULTS
Maximum pimonidazole pixel value and mean pimonidazole labeled fraction was found to be strongly correlated to (18)FDG PET uptake levels, whereas more varying results were obtained for the comparison to (64)Cu-ATSM. In the case of the latter, uptake at scans performed 3 h post injection (pi) generally showed strong positive correlated to pimonidazole uptake.Comparison of distribution patterns of pimonidazole immunohistochemistry and (64)Cu-ATSM autoradiography yielded varying results. Significant positive correlations were mainly found in sections displaying a heterogeneous distribution of tracers.
CONCLUSIONS
Tumors with high levels of pimonidazole staining generally displayed high uptake of (18)FDG and (64)Cu-ATSM (3 h pi.). Similar regional distribution of (64)Cu-ATSM and pimonidazole was observed in most heterogeneous tumor regions. However, tumor and hypoxia level dependent differences may exist with regard to the hypoxia specificity of (64)Cu-ATSM in canine tumors.
背景
本研究旨在比较（64）Cu-二乙酰基双（N（4）-甲基半缩酮）（（64）Cu-ATSM）和（18）FDG PET 摄取特征以及（64）Cu-ATSM 放射自显影与自发犬肉瘤和癌的吡莫硝唑免疫组织化学。
方法
在静脉注射（64）Cu-ATSM 和吡莫硝唑后约 3 至 25 小时内，从单个肿瘤中采集活检。对切片活检进行（64）Cu-ATSM 放射自显影和吡莫硝唑免疫染色。获得的（64）Cu-ATSM 放射自显影和吡莫硝唑图像进行重新缩放、对齐，并比较其分布模式。在一项同时进行的研究中进行了（64）Cu-ATSM 和（18）FDG PET/CT 扫描，并获得了肿瘤的摄取特征。
结果
最大吡莫硝唑像素值和平均吡莫硝唑标记分数与（18）FDG PET 摄取水平密切相关，而与（64）Cu-ATSM 的比较则得到了更多不同的结果。在后一种情况下，注射后 3 小时（pi）扫描的摄取通常与吡莫硝唑摄取呈强烈正相关。吡莫硝唑免疫组织化学和（64）Cu-ATSM 放射自显影分布模式的比较得到了不同的结果。在显示示踪剂异质分布的切片中主要发现了显著的正相关。
结论
高吡莫硝唑染色的肿瘤通常显示出（18）FDG 和（64）Cu-ATSM（3 h pi）的高摄取。在大多数异质肿瘤区域中观察到（64）Cu-ATSM 和吡莫硝唑的相似区域分布。然而，在犬肿瘤中，（64）Cu-ATSM 的缺氧特异性可能存在与肿瘤和缺氧水平相关的差异。
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