Departamento de Química Analítica y Alimentaria, Área de Química Analítica, Facultad de Química, Universidad de Vigo, Campus As Lagoas-Marcosende s/n, 36310 Vigo, Spain.
J Chromatogr A. 2012 Jul 27;1248:48-54. doi: 10.1016/j.chroma.2012.05.092. Epub 2012 Jun 5.
Electromembrane extraction (EME) at low voltage (0-15 V) of 29 different basic model drug substances was investigated. The drug substances with logP<2.3 were not extracted at voltages less than 15 V. Extraction of drug substances with logP≥2.3 and with two basic groups were also effectively suppressed by the SLM at voltages less than 15 V. Drug substances with logP≥2.3 and with one basic group were all extracted at low voltages and with a strong compound selectivity which appeared to have some influence from the polar surface area of the compound. For this group of substances, recoveries varied between 0 and 23% at 5 V, whereas, recoveries varied between 5.5 and 51% at 15 V. Based on mass transfer differences related to charge, polarity, and polar surface, highly selective extractions of drug substances were demonstrated from human plasma, urine, and breast milk. An initial evaluation at low voltage (5 V) was compared with similar extractions at a more normal voltage level (50 V), and this supported that reliable data can be obtained under these low-voltage (mild) conditions by EME.
研究了 29 种不同基本模型药物在低电压（0-15 V）下的电膜萃取（EME）。logP<2.3 的药物在低于 15 V 的电压下不被萃取。logP≥2.3 且具有两个碱性基团的药物在低于 15 V 的电压下也被 SLM 有效抑制。logP≥2.3 且具有一个碱性基团的药物在低电压下均被萃取，且具有很强的化合物选择性，这似乎受到化合物极性表面积的一些影响。对于这组物质，在 5 V 时回收率在 0 到 23%之间变化，而在 15 V 时回收率在 5.5 到 51%之间变化。基于与电荷、极性和极性表面积有关的传质差异，从人血浆、尿液和母乳中证明了对药物的高度选择性萃取。在低电压（5 V）下进行的初步评估与在更正常的电压水平（50 V）下的类似萃取进行了比较，这表明在这些低电压（温和）条件下，通过 EME 可以获得可靠的数据。
