Chemical Engineering Department, Institute of Chemical Technology, Matunga, Mumbai 400 019, India.
Ultrason Sonochem. 2013 Jan;20(1):271-6. doi: 10.1016/j.ultsonch.2012.05.010. Epub 2012 May 29.
This paper illustrates the Ultrasound Assisted Extraction (UAE) of β-carotene from Spirulina platensis. Various parameters such as extraction time, solvent type, biomass to solvent ratio, temperature, electrical acoustic intensity, length of the probe tip dipped into the solvent, duty cycle and pre treatment effect were explored for the extraction of β-carotene. From economic point of view, the optimal conditions for the extraction of β-carotene from Spirulina were 1.5 g Spirulina (2 min pre soaked in methanol) in 50 ml n-heptane at 30 ° C temperature, 167 W/cm(2) electrical acoustic intensity and 61.5% duty cycle for 8 min with probe tip length of 0.5 cm dipped into the extracting solvent from the surface. The maximum extraction achieved under the above mentioned optimum parameters was 47.10%. The pre-treatment time showed a promising effect on the yield as pre-treating the biomass with methanol for 2 min before ultrasonication showed 12 times increase in extraction yield of β-carotene.
本文介绍了从螺旋藻中超声辅助提取β-胡萝卜素的方法。研究了提取时间、溶剂类型、生物量与溶剂比、温度、电功率、探头浸入溶剂的长度、占空比和预处理效果等各种参数对β-胡萝卜素提取的影响。从经济角度考虑，从螺旋藻中提取β-胡萝卜素的最佳条件为：在 30°C 温度下，将 1.5 g 螺旋藻（在甲醇中预浸泡 2 分钟）加入 50 ml 正庚烷中，电功率为 167 W/cm(2)，占空比为 61.5%，探头长度为 0.5 cm，从表面浸入提取溶剂 8 分钟。在上述最佳参数下，最大提取量达到 47.10%。预处理时间对产率有显著影响，超声前用甲醇预处理生物质 2 分钟可使β-胡萝卜素的提取率提高 12 倍。
