Food Safety & Environmental Microbiology Program, Texas A&M University, College Station, TX 77843-2472, USA.
Bioresour Technol. 2012 Aug;118:407-11. doi: 10.1016/j.biortech.2012.05.076. Epub 2012 May 24.
Somatic coliphages are present in high numbers in sewage sludge. Since they are conservative indicators of viruses during wastewater treatment processes, they are being used to evaluate the effectiveness of sludge treatment processes. However, efficient methods to extract them from sludge are lacking. The objective was to compare different virus extraction procedures and develop a method to extract coliphages from sewage sludge. Twelve different extraction buffers and procedures varying in composition, pH, and sonication were compared in their ability to recover indigenous phages from sludges. The 3% buffered beef extract (BBE) (pH 9.0), the 10% BBE (pH 9.0), and the 10% BBE (pH 7.0) with sonication were short-listed and their recovery efficiency was determined using coliphage-spiked samples. The highest recovery was 16% for the extraction that involved 10% BBE at pH 9.0. There is a need to develop methods to extract somatic phages from sludges for monitoring sludge treatment processes.
体噬菌体在污水污泥中数量很高。由于它们是废水处理过程中病毒的保守指标，因此被用于评估污泥处理过程的有效性。然而，从污泥中提取它们的有效方法仍然缺乏。本研究的目的是比较不同的病毒提取程序，并开发一种从污水污泥中提取噬菌体的方法。比较了 12 种不同的提取缓冲液和程序，这些缓冲液和程序在组成、pH 值和超声方面有所不同，以确定它们从污泥中回收土著噬菌体的能力。3%缓冲牛肉提取物（BBE）（pH 9.0）、10% BBE（pH 9.0）和 10% BBE（pH 7.0）加超声被列为候选方法，并使用噬菌体加标样品来确定它们的回收效率。回收率最高的是 10% BBE 在 pH 值为 9.0 时的提取，回收率为 16%。需要开发从污泥中提取体细胞噬菌体的方法，以监测污泥处理过程。
