Smolarek Dorota, Bertrand Olivier, Czerwinski Marcin
Laboratory of Glycoconjugate Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Postepy Hig Med Dosw (Online). 2012 Jun 14;66:348-58. doi: 10.5604/17322693.1000334.
Serum of animals belonging to the Camelidae family (camels and llamas) contains fully active antibodies that are naturally devoid of light chains. Variable domains derived from heavy chain antibodies (hcAb) called VHHs or nanobodies™ can bind antigens as effectively as full-length antibodies and are easy to clone and express. Because of their potential, VHHs are being intensively studied as potential therapeutic, diagnostic and imaging tools. The paper reviews the molecular background of heavy chain antibodies and describes methods of obtaining recombinant fragments of heavy chain antibodies as well as their therapeutic, diagnostic and other applications.
骆驼科动物（骆驼和美洲驼）的血清中含有完全活性的抗体，这些抗体天然不含轻链。源自重链抗体（hcAb）的可变结构域，称为VHH或纳米抗体™，能够像全长抗体一样有效地结合抗原，并且易于克隆和表达。由于其潜力，VHH正作为潜在的治疗、诊断和成像工具被深入研究。本文综述了重链抗体的分子背景，描述了获得重链抗体重组片段的方法及其治疗、诊断和其他应用。
