Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, 410078 China.
Microvasc Res. 2012 Sep;84(2):149-54. doi: 10.1016/j.mvr.2012.06.003. Epub 2012 Jun 15.
Growing interest in utilizing endothelial cells (ECs) for therapeutic purposes has led to the exploration of human embryonic stem cells (hESCs) as a potential source for endothelial progenitors. In this study, ECs were induced from hESC lines and their biological characteristics were analyzed and compared with both cord blood endothelial progenitor cells (CBEPCs) and human umbilical vein endothelial cells (HUVECs) in vitro. The results showed that isolated embryonic KDR+ cells (EC-KDR+) display characteristics that were similar to CBEPCs and HUVECs. EC-KDR+, CBEPCs and HUVECs all expressed CD31 and CD144, incorporated DiI-Ac-LDL, bound UEA1 lectin, and were able to form tube-like structures on Matrigel. Compared with CBEPCs and HUVECs, the expression level of endothelial progenitor cell markers such as CD133 and KDR in EC-KDR+ was significantly higher, while the mature endothelial marker vWF was lowly expressed in EC-KDR+. In summary, the study showed that EC-KDR+ are primitive endothelial-like progenitors and might be a potential source for therapeutic vascular regeneration and tissue engineering.
人们对利用内皮细胞（ECs）进行治疗的兴趣日益浓厚，这促使人们探索人类胚胎干细胞（hESCs）作为内皮祖细胞的潜在来源。在这项研究中，从 hESC 系中诱导出 ECs，并在体外对其与脐血内皮祖细胞（CBEPCs）和人脐静脉内皮细胞（HUVECs）的生物学特性进行了分析和比较。结果表明，分离的胚胎 KDR+细胞（EC-KDR+）表现出与 CBEPCs 和 HUVECs 相似的特征。EC-KDR+、CBEPCs 和 HUVECs 均表达 CD31 和 CD144，摄取 DiI-Ac-LDL，结合 UEA1 凝集素，并能在 Matrigel 上形成管状结构。与 CBEPCs 和 HUVECs 相比，EC-KDR+中内皮祖细胞标志物如 CD133 和 KDR 的表达水平明显更高，而成熟内皮标志物 vWF 的表达水平较低。总之，该研究表明，EC-KDR+是原始的内皮样祖细胞，可能是治疗性血管再生和组织工程的潜在来源。
