Dobretsov G E, Miller Iu I, Kolchin Iu A
Ukr Biokhim Zh (1978). 1990 Sep-Oct;62(5):29-33.
The fluorescent intensity of the N-phenyl-1-amino-8-sulfonaphthalene (ANS) probe significantly decreases in hyperbilirubinemic serum. A decrease of the albumin concentration and absorption of ANS fluorescence by bilirubin cannot explain such a considerable reduction of the probe fluorescence intensity. Measurements of the fluorescence decay kinetics has shown two types of sites occupied by ANS in albumin. ANS quantum yields in hyperbilirubinemic and normal serum are practically identical. The coupling parameters for ANS decrease, but the coupling constant increases under hyperbilirubinemia. As a result the coupling of organic anions with serum albumin significantly decreases if there is high anion concentration, and it does not decrease at low anion concentration. Bilirubin is not a main cause of a decrease of the albumin binding capacity.
N-苯基-1-氨基-8-磺酸萘（ANS）探针在高胆红素血症血清中的荧光强度显著降低。白蛋白浓度的降低以及胆红素对ANS荧光的吸收并不能解释探针荧光强度如此大幅的降低。荧光衰减动力学测量表明，白蛋白中存在两种被ANS占据的位点。高胆红素血症血清和正常血清中的ANS量子产率实际上是相同的。在高胆红素血症情况下，ANS的耦合参数降低，但耦合常数增加。结果是，如果存在高阴离子浓度，有机阴离子与血清白蛋白的耦合会显著降低，而在低阴离子浓度时则不会降低。胆红素不是白蛋白结合能力降低的主要原因。
