Miller Iu I
Lab Delo. 1989(7):20-3.
Quenching of the fluorescence of the N-phenyl-1-amino-8-sulfonaphthalene (ANS) probe in the blood serum of icteric patients is due to not only bilirubin, but more so to other binding inhibitors characteristic of hepatic insufficiency of various etiology. The effects of these inhibitors on ANS binding with albumin may be essentially attenuated if the normal pH values be replaced by pH 4.0. The index, representing the ratio of the ANS fluorescence intensities in the blood serum at pH 7.4 and 4.0, characterizes the serum albumin binding capacity in hepatic insufficiency, and derivation of this index may be used as a clinical laboratory test.
黄疸患者血清中N-苯基-1-氨基-8-磺酸萘（ANS）探针荧光的猝灭不仅归因于胆红素，更主要是由于各种病因导致的肝功能不全所特有的其他结合抑制剂。如果将正常pH值替换为pH 4.0，这些抑制剂对ANS与白蛋白结合的影响可能会显著减弱。代表pH 7.4和4.0时血清中ANS荧光强度比值的指标，表征了肝功能不全时血清白蛋白的结合能力，该指标的推导可作为一项临床实验室检测。
