Miller Iu I, Iushko E G, Dobretsov G E, Krasovitskiĭ B M, Aĭdyraliev R K
Vopr Med Khim. 1990 Mar-Apr;36(2):86-8.
A procedure is developed for estimation of human blood serum albumin in hyperbilirubinemia using a fluorescent probe 9,10-dianiline-2-sulfoanthracene (K-33). Use of the probe N-phenyl-I-amino-8-sulfonaphthalene (ANS) led to underestimation of albumin in hyperbilirubinemia as bilirubin and other inhibitors prevented the ANS binding with blood serum albumin. Substitution of ANS for K-33 enabled to estimate the albumin concentration under conditions of the pathology accompanied by an increase of endogenous ligands content in blood serum. The rate of K-33 (15 mM) fluorescence in blood serum (diluted 400-fold, at pH 4.0) correlated with albumin concentration (r-0.955).
开发了一种使用荧光探针9,10 - 二苯胺 - 2 - 磺基蒽（K - 33）测定高胆红素血症患者血清白蛋白的方法。使用探针N - 苯基 - I - 氨基 - 8 - 磺基萘（ANS）会导致高胆红素血症患者血清白蛋白的测定值偏低，因为胆红素和其他抑制剂会阻止ANS与血清白蛋白结合。用K - 33替代ANS能够在伴有血清中内源性配体含量增加的病理状况下测定白蛋白浓度。血清中（稀释400倍，pH 4.0）K - 33（15 mM）的荧光速率与白蛋白浓度相关（r = 0.955）。
