可注射的原位物理化学交联的结冷胶水凝胶。
Injectable in situ physically and chemically crosslinkable gellan hydrogel.
机构信息
Department of Surgery and Medical Research, Veteran Affairs Medical Center, St. Louis, MO 63106, USA.
出版信息
Macromol Biosci. 2012 Jul;12(7):952-61. doi: 10.1002/mabi.201100422. Epub 2012 Jun 18.
Abstract
An injectable, in situ physically and chemically crosslinkable gellan hydrogel is synthesized via gellan thiolation. The thiolation does not alter the gellan's unique 3-D conformation, but leads to a lower phase transition temperature under physiological conditions and stable chemical crosslinking. The synthesis and hydrogels are characterized by (1)H NMR, FT-IR, CD, or rheology measurements. The injectability and the tissue culture cell viability is also tested. The thiolated gellan hydrogel exhibits merits, such as ease for injection, quick gelation, lower gelling temperature, stable structure, and nontoxicity, which make it promising in biomedicine and bioengineering as an injectable hydrogel.
摘要
一种可注射的、原位物理化学交联的结冷胶水凝胶是通过结冷胶巯基化合成的。巯基化不会改变结冷胶独特的 3D 构象，但会导致在生理条件下更低的相转变温度和稳定的化学交联。该合成物和水凝胶通过（1）H NMR、FT-IR、CD 或流变学测量进行了表征。还测试了其可注射性和组织培养细胞活力。巯基化结冷胶水凝胶具有易于注射、快速凝胶化、较低的凝胶化温度、稳定的结构和无毒性等优点，使其有望在生物医学和生物工程领域作为一种可注射水凝胶得到应用。
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